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RT-LAMP Fluorenscent Master Mix HCB5205A Sawirka Sifaysan
  • RT-LAMP Fluorescent Master Mix HCB5205A

RT-LAMP Fluorescent Master Mix


Bisadda No: HCB5205A

Xidhmada: 96RXN/960RXN/9600RXN

Alaabtani waxay ka kooban tahay falcelinta falcelinta, RT-Enzymes Mix (Bst DNA polymerase and transcriptase reverse kulaylka), Ilaaliyeyaasha lyophilized iyo qaybaha dheeha koromojiinka.

Sharaxaada Alaabta

Faahfaahinta alaabta

Alaabtani waxay ka kooban tahay falcelinta falcelinta, RT-Enzymes Mix (Bst DNA polymerase and transcriptase reverse kulaylka), Ilaaliyeyaasha lyophilized iyofluorescentqaybaha dheeha.Badbaadiyaha falcelinta ayaa ka kooban Mg2+, dNTP iyo qaybaha kale ee lagama maarmaanka u ah xoojinta.Si aad u isticmaasho, kaliya ku qas Buffer, falcelinta enzyme, dheeha fluorescent, primer oo ku dar template, ku darida ilaalinta lyophilized ayaa si toos ah u isticmaali karta.Waxay ku xidhnayd lyophilizer iyo lyophilized, oo kaliya furayaasha iyo qaababka ayaa lagu daray markii la isticmaalay.Reagent-kaani waxa uu leeyahay hufnaan sare u qaadis iyo dareen


  • Hore:
  • Xiga:

  • Qayb

    Qayb

    HCB5205A-01

    HCB5205A-02

    HCB5205A-03

    Kordhinta Kordhinta Loop-dhexdhexaadiyay

    1.2 ml

    4 ml×3

    12 ml×10

    RT-Enzymes Isku-dhafka

    245 μl

    2.45 ml

    2.45 ml×10

    Difaaca lyophilized

    0.96 ml×2

    9.60ml×2

    9.60ml×20

    25× Midab

    192 μl

    0.96 ml×2

    0.96 ml×20

     

    Codsiyada

    Si loo kordhiyo DNA-ga ama RNA isothermal.

     

    Xaaladaha Kaydinta

    Lagu raray baraf qallalan, oo lagu kaydiyay -25 ~ -15 ℃.Iska ilaali baraf-dhalaalid joogto ah, alaabtu waxay shaqaynaysaa 12 bilood.

     

    Baratakoolka

    1.Ku dhalaali furaha falcelinta si loogu isticmaalo heerkulka qolka.Vortex si kooban ama u rogo tuubooyinka dhawr jeer si aad si fiican isugu walaaqdo, ka dibna sentrifuge si aad dareeraha ugu ururiso xagga hoose ee tuubada.

    2.Diyaarinta habka falcelinta.Reagent-kan waxaa lagu diyaarin karaa laba hab oo falcelineed, isku darka falcelinta dareeraha ah iyo isku dhafka nidaamka lyophilized.

    1) Diyaari isku dhafka falcelinta dareeraha

    Qayb

    Mugga

    Kordhinta Kordhinta Loop-dhexdhexaadiyay

    12.5 μl

    25× Midab

    2 μL

    RT-Enzymes Isku-dhafka

    2.55 μl

    10 × Isku dhafka koowaada

    5 μL

    Qaababka DNA/RNA b

    × μL

    Biyo aan Nukliyeer lahayn

    Ilaa 50 μl

     

    2) Isku darka nidaamka Lyophilization

    ① Diyaari isku darka lyophilized

    Qayb

    Mugga

    Kordhinta Kordhinta Loop-dhexdhexaadiyay

    12.5 μl

    Difaaca lyophilized

    20 μL

    25× Midab

    2 μL

    RT-Enzymes Isku-dhafka

    2.55 μl

    Biyo aan Nukliyeer lahayn

    Ilaa 50 μl

    ② Lyophilization: Isku-darka la diyaariyey waxaa lagu lyophilized nidaamka 50μL

    ③ Diyaarso isku darka falcelinta

    Qayb

    Mugga

    Isku darka Lyophilized

    1 gabal

    10 × Isku dhafka koowaada

    5 μL

    Qaababka DNA/RNA b

    × μL

    Biyo aan Nukliyeer lahayn

    Ilaa 50 μl

    Xusuusin:

    1) ah.10×Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;

    2) b.DEPC (biyaha ku milma) ayaa lagula talinayaa templ nucleic acid.

    3)Falcelinta Kordhinta: Ku deji barnaamijka soo socda qalabka PCR-ga ee fluorescent (tusaale ABI7500, iwm.):

    Heerkulka

    Waqtiga

    Goobo

    65 ℃

    60 ilbiriqsi * ururi fluorescence FAM

    30

     

    Xusuusin

    1.milixdu waxa ay ka soo muuqan kartaa gunta hoose ee tuubada, vortex si kooban ama tuubooyinka roganaya dhawr jeer si si fiican loogu qaso heerkulka qolka.

    2.Heerkulka falcelinta waxaa lagu hagaajin karaa inta u dhaxaysa 62 ℃ iyo 68 ℃ iyadoo loo eegayo xaaladda asaasiga ah.

    3.Tijaabada waa in la jaangooyaa, oo ay ku jiraan diyaarinta habka falcelinta, lyophilization, iyo habraaca muunad samaynta iyo muunad kordhinta;

    4.Si looga fogaado wasakheynta, waxaa lagu talinayaa in lagu diyaariyo habka falcelinta ee kursiga keydka aadka u nadiifka ah, kuwa kale Ku dar qaabyaalo daboolka qiiqa ee qolka si looga fogaado faragelin been ah.

     

     

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