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Superstart Taq DNA Polymerase HC1013A Sawirka Featured
  • Superstart Taq DNA Polymerase HC1013A

Superstart Taq DNA Polymerase


Bisadda No: HC1013A

Xidhmada: 0.1ml/1ml/5ml

Superstart Taq DNA Polymerase waa bilow kulul oo polymerase DNA ah.

Sharaxaada Alaabta

Faahfaahinta alaabta

Superstart Taq DNA Polymerase waa bilow kulul oo polymerase DNA ah.Alaabtani ma aha oo kaliya in ay si fiican u hor istaagto falcelinta aan khaaska ahayn ee ay keento ciribtirka aan gaarka ahayn ee furayaasha ama isugaynta asaasiga ah ee habka diyaarinta iyo kordhinta nidaamka PCR.Sidaa darteed, waxay ku gaari kartaa natiijooyin ka wanaagsan xoojinta badan.Sidoo kale, waxay wanaajin kartaa kor u qaadida qaababka foojignaanta hoose si loo helo qadar sare oo badeecad ah oo loo gaaro saameyn xasilloon oo aad u weyn.


  • Hore:
  • Xiga:

  • Qaybaha

    1.5 U/μL Superstart Taq DNA polymerase

    2.10 × PCR Buffer II (Mg²+ bilaash ah) (ikhtiyaar)

    3.25 mm MgCl2(ikhtiyaar)

    * 10 × PCR Buffer II (Mg²+ bilaash ah) kuma jiraan dNTP iyo Mg²+, fadlan ku dar dNTPs iyo MgCl2marka la diyaarinayo habka falcelinta.

     

    Codsiyada lagu taliyay

    1.ogaanshaha qandhada doofaarka ee Afrika.

    2.Ogaanshaha STI

    3.Kordhinta Multiplex.

     

    Xaalada Kaydinta

    -20°C kaydinta muddada dheer, waa in si fiican loo qaso ka hor inta aan la isticmaalin, iska ilaali baraf-dhalaalid joogto ah.

    *Haddii roobabku dhacaan qaboojiyaha ka dib, waa caadi;waxaa lagu talinayaa in la is waafajiyo heerkulka qolka ka hor inta aan la qasin oo la isticmaalin.

     

    Qeexitaan Unug

    Hal unug firfircoon (U) ayaa lagu qeexaa sida qadarka ensaymka ee ku dara 10 nmol of deoxyribonucleotide galay walxo aashito- milmi kara at 74°C muddo 30mins ah iyadoo la isticmaalayo shahwada salmon firfircoon DNA sida template/primer.

     

    Xakamaynta tayada

    1.SDS-PAGE nadiifinta korantada ee ka weyn 98%.

    2.Dareenka weynaynta, xakamaynta dufcaddii, xasilloonida.

    3.Wax dhaqdhaqaaq nuclease ah oo dibadda ah ma jiro, ma jiro endonuclease exogenous ama faddarayn exonuuclease

     

    Tilmaamaha

    Dejinta falcelinta

    Qaybaha

    Mugga (μL)

    U-fiirsashada kama dambaysta ah

    10 × PCR Buffer II (Mg²+ bilaash ah)a

    5

    dNTPs (10mM dNTP kasta)

    1

    200 μM

    25 mm MgCl2

    2-8

    1-4 mm

    Superstart Taq DNA Polymerase (5U/μL)

    0.25-0.5

    1.25-2.5 U

    25 × Isku dhafka koowaadb 

    2

    Template

    -

    1 μg/ falcelin

    ddH2O

    Ilaa 50

    -

    Xusuusin:

    1) ah.Kaydka kuma jiraan dNTP iyo Mg²+, fadlan ku dar dNTPs iyo MgCl2marka la diyaarinayo habka falcelinta.

    2) b.Haddii loo isticmaalo qPCR/qRT-PCR, shaybaarrada fluorescent waa in lagu daraa habka falcelinta.Caadi ahaan, fiirsashada ugu dambeysa ee 0.2 μM waxay siin doontaa natiijooyin wanaagsan;haddii waxqabadka falcelinta uu liito, xooga saarista asaasiga ah waxaa lagu hagaajin karaa inta u dhaxaysa 0.2-1 μM.Xoojinta baaritaanka ayaa ah

    3) inta badan waxaa lagu hagaajiyay inta u dhaxaysa 0.1-0.3 μM.Tijaabooyin isugeyn xoog leh ayaa la sameyn karaa si loo helo isku dhafka ugu wanaagsan ee asaasiga ah iyo baaritaanka.

     

    Nidaamka baaskiil wadida kulaylka

    Habka laba-tallaabo

    Talaabada

    Heerkulka

    Waqtiga

    Wareegtada

    Denaturation ka hor

    95℃

    1-5 daqiiqo

    1

    Denaturation

    95℃

    10-20 ilbiriqsi

    35-50

    Annealing / Kordhinta

    56-64℃ 

    20-60 ilbiriqsi

     

    Thoos-tallaabo

    Talaabada

    Heerkulka

    Waqtiga

    Wareegtada

    Denaturation ka hor

    95℃

    1-5 daqiiqo

    1

    Denaturation

    95℃

    10-20 ilbiriqsi

    35-50

    Annealing

    56-64℃

    10-30 ilbiriqsi

    Kordhinta

    72℃

    10-60 sekan

     

    Xusuusin

    1.Waxay qaadan kartaa 95 ℃ ama 94 ℃, 1 ~ 5 daqiiqo kuleyl degdeg ah.

    2.Nidaamku aad buu u la qabsan karaa, oo leh gaar gaar ah iyo dareen sare.

    3.PCR-ga caadiga ah, waxa ay ku heli kartaa xaddi badan oo badeeco ah, iyo marka la eego fluorescence quantitative PCR, waxa ay wanaajin kartaa sida caadiga ah ee qalooca cod-weyneeye iyo qiimaha fluorescence ee templateka oo leh feejignaan aad u hooseeya.Ku haboon in loo isticmaalo sidii dareen sare oo PCR ah reagent ogaanshaha.

    4.waxaana loo isticmaali karaa falcelinta kor u qaadista PCR multix.

    5.5′→3′ firfircoonida polymerase,5′→3′ dhaqdhaqaaqa exonuclease;maya 3′→5′ dhaqdhaqaaqa exonuclease;ma laha shaqo saxid.

    6.Ku habboon baaritaanka tayada iyo tirada PCR iyo RT-PCR.

    7.Dhamaadka 3′ ee badeecada PCR waa A, kaas oo si toos ah loogu xidhi karo T vector.

    8.Habka saddex-tallaabo ayaa lagu talinayaa in loogu talagalay curiyeyaasha heerkulku hooseeyo ama si loo kordhiyo jajabyada ka badan 200 bp.

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