
Taqman Multiplex qPCR Premix
Bisadda No: HCB5061B
Taqman Multiplex qPCR Premix Master mix waa jiil cusub oo ka hortag ah oo wax laga beddelay Taq DNA Polymerase fluroescence quantitative qPCR waxaa loo isticmaali karaa reagent lyophilization.Waxay ku haboon tahay FAM/HEX/TET/JOE/ROX iwmReagent-ku waxa uu ka kooban yahay hotstart Fast Taq DNA Polymerase, dNTP iwm. Qayb si bilaash ah loogu qallajin karo mishiinka.Alaabta kuma jiraan midabada la saxay ee ROX waxayna ku habboon tahay noocyo kala duwan oo PCR ah.
Qaybaha
Lyo-Diyaar u ah TaqMan qPCR Mix
Xaaladaha Kaydinta
Alaabtan waa in lagu kaydiyaa meel -25~-15℃ ka fog iftiinka muddo 1 sano ah.
Tilmaamaha
1.Habka falcelinta
Qaybaha | Mugga (μL) | U fiirsashada kama dambaysta ah |
Isku qas Enzyme | 20 | - |
Primer Primer (10μM) | x | 0.1-0.5μM |
Baadhista Hore (10μM) | x | 50-250nM |
DNA Tamplate | 1-25 | - |
dd H2O | ilaa 25 | - |
2.Barnaamijka falcelinta
Tallaabo wareeg | Heerkulka | Waqtiga | Wareegtada |
Denaturation bilowga ah | 95℃ | 5 daqiiqo | 1 |
Falcelinta weynaynta | 95℃ | 15 ilbiriqsi | 45 |
Baabi'inta/kordhinta | 60℃ | 30 ilbiriqsi |
Xusuusin:Xaalado gaar ah heerkulka nuugista/kordhinta waxaa lagu hagaajin karaa iyadoo loo eegayo qiimaha asaasiga ah ee Tm, 60°C ayaa lagula talinayaa.
Habka Lyophilization
Habraaca | Heerkulka | Waqti deji | Waqtiga muddada | Cadaadis |
Horay u qaboojin | -45 ℃ | 10min | 60min | - |
Qalajinta aasaasiga ah | -45 ℃ | 10min | 60min | 0.01mbar |
-40℃ | 10min | 600min | 0.01mbar | |
-35 ℃ | 10min | 120min | 0.01mbar | |
-30℃ | 20 daqiiqo | 60min | 0.01mbar | |
-20℃ | 20 daqiiqo | 60min | 0.01mbar | |
-10℃ | 20 daqiiqo | 60min | 0.01mbar | |
0℃ | 20 daqiiqo | 60min | 0.01mbar | |
Qallajinta sare | 5℃ | 10min | 20 daqiiqo | 0.01mbar 0.01mbar |
10℃ | 10min | 20 daqiiqo | ||
15℃ | 10min | 20 daqiiqo | 0.01mbar | |
20℃ | 10min | 20 daqiiqo | 0.01mbar | |
25℃ | 10min | 240min | 0.01mbar |
Xusuusin
1. Alaabtan waxaa loogu talagalay isticmaalka cilmi-baarista oo keliya.
2. Fadlan ku shaqee jaakadaha shaybaadhka iyo galoofyada la tuuri karo, badbaadadaada awgeed.