Hotstart Taq DNA Polymerase (5u/ul)
Taq DNA Polymerase waa bilawga kulul ee DNA polymerase oo leh labajibbaaran xannibaado labajibbaaran unugyada difaaca jirka. Alaabtani kaliya ma xannibayso 5′ 3′ polymerase dhaqdhaqaaqa Taq DNA polymerase, laakiin sidoo kale waxay xannibaysaa dhaqdhaqaaqa 5′→3′exonuclease.Kuleylinta 30 ilbiriqsi heerkulka ka hor denaturation waxay si buuxda u dhaqaajin kartaa antibody waxayna sii deyn kartaa dhaqdhaqaaqa DNA polymerase iyo dhaqdhaqaaqa exonuclease.Sifada xannibaadda labanlaabka ah kaliya si wax ku ool ah ugama hor istaagi karto kordhinta aan gaarka ahayn ee ay keento is-dheelli-tir la'aanta ama dimer-ka aasaasiga ah, laakiin sidoo kale waxay si wax ku ool ah u joojin kartaa hoos-u-dhaca calaamadda fluorescence ee ay keento hoos u dhaca baaritaanka, si looga dhigo reagent ogaanshaha in vitro mid xasilloon inta lagu jiro gaadiidka ama isticmaalka qolka. heerkulka.
Qaybaha
| Qayb | HC1012B (250U) | HC1012B (1000U) | HC1012B (10000U) | HC1012B (25000U) |
| Taq DNA Polymerase(5 U/μL) | 50 μL | 200 μL | 2 ml | 5 ml |
Xaalada Kaydinta
Alaabta waxaa lagu raray baraf qallalan waxaana lagu kaydin karaa -25°C~-15°C muddo 2 sano ah.
Tilmaamaha
| Polymerase | Taq DNA Polymerase |
| daahirnimo | ≥ 95% (SDS-PAGE) |
| Bilow kulul | Ku-dhismay Hot Start |
| Xawaaraha falcelinta | Heerka |
| Waxqabadka Exonuclease | 5′→3′′ |
Tilmaamaha
Dejinta falcelinta
| Qaybaha | Mugga (μL) | U-fiirsashada kama dambaysta ah |
| 2× kaydkaa | 25 | 1× |
| Isku darka aasaasiga ah / Baadhitaankab | × | 0.1 μmol/L-0.5 μmol/L |
| Hotstart Taq Polymerase (5U/μL) | 1.2 | 0.12 U/μL |
| Qaabka DNA-dac | × | 0.1-100 ng |
| ddH2O | Ilaa 50 | - |
Xusuusin:
1) Marka loo eego codsiga tijaabada ah ee gaarka ah, waxaa loo baahan yahay in la diyaariyo falcelinta falcelinta u dhiganta.
2) Qadarka DNA-da iyo fiirsashada baarayaasha ama aasaasayaasha ayaa lagu talinayaa in la uruuriyo.Xoojinta ugu fiican ayaa lagu hagaajin karaa iyadoo loo eegayo xaaladaha tijaabada gaarka ah.
Nidaamka baaskiil wadida kulaylka
| Talaabada | Heerkulka(°C) | Waqtiga | Wareegtada |
| Denaturation ka hor | 95 ℃ | 5 daqiiqo | 1 |
| Denaturation | 95 ℃ | 15 ilbiriqsi | 45 |
| Annealing / Kordhinta | 60 ℃a | 30 ilbiriqsib |
Xusuusin:
1) Heerkulka falcelinta waxaa lagu hagaajiyaa iyadoo loo eegayo qiimaha Tm ee aasaasayaasha loo qaabeeyey.
2) Qalab kala duwan oo qPCR ah waxay u baahan yihiin wakhtiga helitaanka calaamada fluorescence oo kala duwan, fadlan u deji si waafaqsan wakhtiga ugu gaaban.
Xusuusin
Fadlan xidho PPE-ga lagama maarmaanka ah, sida jaakada shaybaadhka iyo galoofyada, si loo hubiyo caafimaadkaaga iyo badbaadadaada!
