Qalabka Baaritaanka Hal Tilaabo ee Multiplex RT-qPCR (UDG Plus)
Sharaxaada
Multiplex One Tallaabo RT-qPCR Kit (UDG Plus) waa qalab baadhitaan ku salaysan RT-qPCR si degdeg ah oo ku habboon hal-plex ama PCR tiro badan oo badan iyadoo la isticmaalayo RNA qaab ahaan (sida fayraska RNA).Waxaa loo adeegsadaa in lagu xoojiyo DNA-ga laba-jibbaaran ee qaab-dhismeedka RNA-ga hal-xidhaale leh si loogu oggolaado qiyaas-dhakhso-waqtiga dhabta ah ee bartilmaameedyada RNA.Talaabada qoraalka ah ee gadaale transcriptase-ku waxay soo saartaa unugyada DNA-da ee hal-xidhaaleed (cDNA) oo kaabaya qaabka RNA.
Habka falcelinta
Qaybaha
| Qaybaha | 200rxns | 1,000rns | 10,000 rxn |
| RNase-free ddH2O | 3*1ml | 15ml | 150ml |
| 5*hal tallaabo isku darka | 800ml | 4*1ml | 40ml |
| Hal tallaabo oo isku darka enzyme | 200ml | 1ml | 10ml |
| 50* ROX tixraaca dheeha 1 | 80ml | 400ml | 4*1ml |
| 50* ROX tixraaca dheeha 2 | 80ml | 400ml | 4*1ml |
a.Hal-tallaabo Buffer waxa ku jira dNTP Mix iyo Mg2+.
b.Isku-dhafka Enzyme wuxuu inta badan ka kooban yahay transcriptase-ka-noqoshada, Hot Start Taq DNA polymerase (wax-ka-beddelka unugyada difaaca) iyo horjoogaha RNase.
c.Loo isticmaalo in lagu saxo qaladka sinals fluorescene inta u dhaxaysa ceelasha kala duwan.
c.ROX: Waxaad u baahan tahay inaad doorato jaangooynta iyadoo loo eegayo qaabka qalabka tijaabada.
Codsiyada
Ogaanshaha cudur-sidaha
ogaanshaha iyo cilmi baarista burada
Ogaanshaha cudurka xoolaha
Ogaanshaha hore ee cudurrada la iska dhaxlo
Ogaanshaha bakteeriyada cudur-sidaha ee cuntada
Raridda iyo Kaydinta
Gaadiidka:Xirmooyinka barafka
Xaaladaha Kaydinta:Ku kaydi at -30 ~ -15 ℃.
Nolosha Shief:1 sano
