Hal Tallaabo xirmada baaritaanka ee RT-qPCR
Sharaxaada
U+ Hal Tilaabo RT-qPCR Kit (Glycerol-free) waa hal-tallaabo RT-qPCR reagent glycerol-la'aan ah iyadoo la isticmaalayo RNA qaab template ah (sida fayraska RNA), kaas oo ku habboon horumarinta iyo naqshadeynta alaabta lyophilized.Alaabtani waxay midaysaa waxqabadka sare ee hal-tallaabo u heellan Reverse Transcriptase iyo bilawga kulul ee Champagne Taq DNA Polymerase, oo leh kayd kiimiko qallajin si wanaagsan loo hagaajiyay, kaas oo leh hufnaan, dheellitirnaan iyo gaar ahaan, oo la jaan qaadaya qaboojinta-qallajinta kala duwan. Intaa waxaa dheer, nidaamka ka-hortagga wasakhda dUTP / UDG waxaa lagu soo bandhigay reagent, kaas oo ka shaqayn kara heerkulka qolka, baabi'in kara saamaynta faddaraynta alaabta qPCR, iyo in la hubiyo saxnaanta natiijooyinka.
Mabaadi'da aasaasiga ah ee RT-qPCR
Tilmaamid
| Walxaha Tijaabada | Tilmaamid | Natiijo |
| (SDS PAGE) Nadiifinta Kaydka Enzyme (SDS PAGE) | ≥95% | Gudub |
| Dhaqdhaqaaqa Endonuclease | Lama ogaan | Gudub |
| Dhaqdhaqaaqa Exodulease | Lama ogaan | Gudub |
| Waxqabadka Rnase | Lama ogaan | Gudub |
| Haraaga E.coli DNA | 1 koobi/60 | Gudub |
| Qaabka Asay-System | 90%≤110% | Gudub |
Qaybaha
| Qaybaha | 100rxns | 1,000rns | 5,000 rxn |
| RNase-free ddH2O | 2*1ml | 20ml | 100ml |
| 5*hal tallaabo isku darka | 600ml | 6*1ml | 30ml |
| Hal tallaabo oo isku darka enzyme | 150ml | 2*750ml | 7.5ml |
| 50* ROX tixraaca dheeha 1 | 60ml | 600ml | 3*1ml |
| 50* ROX tixraaca dheeha 2 | 60ml | 600ml | 3*1ml |
a.Hal-tallaabo Buffer waxa ku jira dNTP Mix iyo Mg2+.
b.Isku-dhafka Enzyme wuxuu inta badan ka kooban yahay gadaal
transcriptase, Hot Start Taq DNA polymerase (wax ka beddelka antibody) iyo horjoogaha RNase.
c.Loo isticmaalo in lagu saxo qaladka sinals fluorescene inta u dhaxaysa ceelasha kala duwan.
c.ROX: Waxaad u baahan tahay inaad doorato jaangooynta iyadoo loo eegayo qaabka qalabka tijaabada.
Codsiyada
ogaanshaha QPCR
Raridda iyo Kaydinta
Gaadiidka:Xirmooyinka barafka
Xaaladaha Kaydinta:Ku kaydi at -20℃.
Shief Life:18 bilood
