RT-LAMP Fluorescent Master Mix (Kuul Layfilay)
Sharaxaada Alaabta
LAMP hadda waxaa si weyn loo isticmaalaa tignoolajiyada xagga sare-u-qaadista isothermal.Waxay isticmaashaa 4-6 asal oo tilmaami kara 6 gobol oo gaar ah oo ku saabsan hidda-wadaha bartilmaameedka ah, waxayna ku tiirsan tahay dhaqdhaqaaqa barokaca xooggan ee Bst DNA polymerase.Waxaa jira habab badan oo lagu ogaado LAMP, oo ay ku jiraan habka dheeha, habka midabaynta pH, habka turbidity, HNB, calcein, iwm. RT-LAMP waa hal nooc oo falcelinta LAMP oo leh RNA qaab ahaan.RT-LAMP Fluorescent Master Mix (Powder Lyophilized) waxay ku jirtaa qaabka budada Lyophilized, oo kaliya waxay u baahan tahay inay ku darto furayaasha iyo qaababka marka la isticmaalayo.
Sheegid
| Walxaha tijaabi | Tilmaamaha |
| Endonulease | Lama dooran |
| Dhaqdhaqaaqa RNase | Midna lama ogaan |
| Dhaqdhaqaaqa DNA-ga | Midna lama ogaan |
| Dhaqdhaqaaqa Nickase | Midna lama ogaan |
| E. koligDNA | ≤10 koobi/500U |
Qaybaha
Alaabtani waxa ay ka kooban tahay Reaction Buffer, RT-Enzymes Isku darka Bst DNA Polymerase iyo Thermostable Reverse Transcriptase, Lyoprotectant iyo Walxaha Dheelaha Fluorescent.
Amplication
Kordhinta Isothermal ee DNA iyo RNA.
Dhoofinta iyo Kaydinta
Gaadiidka:Ambient
Xaaladaha Kaydinta:Ku kaydi at -20℃
Taariikhda dib-u-imtixaanka lagu taliyay:18 bilood
