Qalabka tijaabada Glycohemoglobin A1c (HbA1c).
Faa'iidooyinka
● Saxnaanta sare
● Awood xoog leh oo ka-hortagga faragelinta
● Degenaansho wanaagsan
Qaab-dhismeedka Kiimikada
Codsiyada
Tijaabada in vitro ee go'aaminta tirada ee HbA1c ee dhiiga bini'aadamka oo dhan ee hababka sawir-qaadista.HbA1c waa wax soo saarka hemoglobin (Hb) kaas oo soo saara falcelin gaabis ah oo joogto ah oo aan ahayn enzymatic glycation hoos gulukoos dhiig oo sarreeya.Gulukoosku wuxuu wax ka beddelaa hemoglobin gaar ahaan hadhaaga valine n-terminal si uu u sameeyo haemoglobin glycated.Marka la eego xaaladaha jireed ee caadiga ah, soo saarista alaabada falcelinta glycosylation ee aan enzymatic ahayn ayaa si togan u dhigma fiirsashada falcelinta.Maaddaama xoogga haemoglobin-ku uu yahay mid deggan, heerarka glycosylation waxay inta badan ku xiran yihiin fiirsashada gulukooska waxayna sidoo kale la xiriiraan dhererka haemoglobin iyo soo-gaadhista gulukoosta.Sidaa darteed, HbA1c waxay tilmaame wanaagsan u tahay celceliska heerka gulukooska dhiigga ee bukaannada 2-3 bilood ee u dambeeya.
Mabda'a
Marka la eego ficilka protease, n-terminal ee silsiladda β ee HbA1c waa la gooyaa waxaana la sii daayaa dipeptides glycosylated.Dareen-celinta ugu horreysa, xoojinta Hb waxaa lagu heli karaa iyadoo la cabbirayo nuugista 480 nm.Dareen-celinta labaad, fructosyl peptide oxidase (FPOX) waxay ku shaqeysaa dipeptides glycosylated si ay u abuurto hydrogen peroxide kaas oo ka falcelin kara wakiilada chromogenic si ay u dhaliyaan nuugista 660nm joogitaanka peroxidase, ka dibna diiradda HbA1c waxaa lagu heli karaa iyadoo la cabbirayo nuugista 660nm.Marka loo eego fiirsashada HbA1c ee la helay iyo feejignaanta Hb, boqolkiiba HbA1c(HbA1c%) waa la xisaabin karaa.
Lagu dabaqi karo
Hitachi 7180/7170/7060/7600 falanqeeyaha biochemical ee tooska ah, Abbot 16000, OLYMPUS AU640
Reagents
| Qaybaha | Ufiirsasho |
| Reagent 1 (R1) | |
| Badbaadiyaha wanaagsan | 100mmol/L |
| PRK | 500KU/L |
| DA-67 | 10mmol/L |
| Reagents 2 (R2) | |
| Badbaadiyaha wanaagsan | 100mmol/L |
| Fructosyl peptide oxidase | 50 KU/L |
| Reagent 3 (R3) | |
| Badbaadiyaha wanaagsan | 100mmol/L |
Gaadiidka iyo kaydinta
Gaadiidka:Ambient
Kaydinta iyo Deganaanshaha:
Ilaa taariikhda uu dhacayo ee lagu tilmaamay calaamadda, marka lagu kaydiyo iyada oo aan la furin 2-8℃ lagana ilaaliyo iftiinka.Marka la furo, reagents-yadu waxay xasilan yihiin 28 maalmood marka la qaboojiyo falanqeeyaha ama qaboojiyaha.
Wasakhaynta reagents waa in laga fogaadaa.Ha baraf gelinin reageniyeyaasha.
Marka la kala diro, calibrator-ku wuu xasiloon yahay 15 maalmood 2–8 ℃, koontaroolkuna wuu xasiloon yahay 7 maalmood 2–8℃, ha bafin.
Nolosha shelf1 sano
