Hal Tallaabo xirmada baaritaanka ee RT-qPCR

Hal Tilaabo qRT-PCR Probe Kit waxaa si gaar ah loogu talagalay qPCR kaas oo si toos ah u isticmaala RNA (tusaale fayraska RNA) sida template.Isticmaalka asal-raacyada gaarka ah ee hidda-wadaha (GSP), qoraalka gadaasha iyo qPCR waxaa lagu dhammeyn karaa hal tubo, taasoo si weyn u yareyneysa hababka tuubooyinka iyo khatarta wasakheynta.Waxaa lagu dami karaa at 55 ℃ iyada oo aan saameyn ku yeelan hufnaanta iyo dareenka qRT-PCR.Isku darka waxqabadka sare ee HiScript III Reverse Transcriptase iyo bilawga kulul ee Champagne Taq DNA Polymerase, oo leh nidaam la hagaajiyay, dareenka ogaanshaha Hal Tallaabo qRT-PCR Probe Kit wuxuu gaari karaa 0.1 pg wadarta RNA ama in ka yar 10 nuqul oo qaab-dhismeedka RNA ah. waxayna leedahay xasilooni kulayl sare leh.Hal Tilaabo qRT-PCR Kit Probe Kit waxa lagu bixiyaa Master Mix.Isku darka 5 × Hal Tallaabo waxa uu ka kooban yahay kayd la hagaajiyay iyo dNTP/dUTP Mix, oo ku habboon hababka ogaanshaha gaarka ah ee ku salaysan shaybaarrada summadaysan (tusaale TaqMan).