Hal Tallaabo xirmada baaritaanka ee RT-qPCR
Sharaxaada
Hal Tilaabo qRT-PCR Probe Kit waxaa si gaar ah loogu talagalay qPCR kaas oo si toos ah u isticmaala RNA (tusaale fayraska RNA) sida template.Isticmaalka asal-raacyada gaarka ah ee hidda-wadaha (GSP), qoraalka gadaasha iyo qPCR waxaa lagu dhammeyn karaa hal tubo, taasoo si weyn u yareyneysa hababka tuubooyinka iyo khatarta wasakheynta.Waxaa lagu dami karaa at 55 ℃ iyada oo aan saameyn ku yeelan hufnaanta iyo dareenka qRT-PCR.Hal Tilaabo qRT-PCR Kit Probe Kit waxa lagu bixiyaa Master Mix.Isku darka 5 × Hal Tallaabo waxa uu ka kooban yahay kayd la hagaajiyay iyo dNTP/dUTP Mix, oo ku habboon hababka ogaanshaha gaarka ah ee ku salaysan shaybaarrada summadaysan (tusaale TaqMan).
Mabaadi'da aasaasiga ah ee RT-qPCR
qeexid
| Walxaha Tijaabada | Tilmaamid | Natiijo |
| (SDS PAGE) Nadiifinta Kaydka Enzyme (SDS PAGE) | ≥95% | Gudub |
| Waxqabadka Endonuclease | Lama ogaan | Gudub |
| Dhaqdhaqaaqa Exodulease | Lama ogaan | Gudub |
| Waxqabadka Rnase | Lama ogaan | Gudub |
| Haraaga E.coli DNA | 1 koobi/60 | Gudub |
| Qaabka Asay-System | 90%≤110% | Gudub |
Qaybaha
| Qaybaha | 100rxns | 1,000rns | 5,000 rxn |
| RNase-free ddH2O | 2*1ml | 20ml | 100ml |
| 5*hal tallaabo isku darka | 600ml | 6*1ml | 30ml |
| Hal tallaabo oo isku darka enzyme | 150ml | 2*750ml | 7.5ml |
| 50* ROX tixraaca dheeha 1 | 60ml | 600ml | 3*1ml |
| 50* ROX tixraaca dheeha 2 | 60ml | 600ml | 3*1ml |
a.Hal-tallaabo Buffer waxa ku jira dNTP Mix iyo Mg2+.
b.Isku-dhafka Enzyme wuxuu inta badan ka kooban yahay gadaal
transcriptase, Hot Start Taq DNA polymerase (wax ka beddelka antibody) iyo horjoogaha RNase.
c.Loo isticmaalo in lagu saxo qaladka sinals fluorescene inta u dhaxaysa ceelasha kala duwan.
d.ROX: Waxaad u baahan tahay inaad doorato jaangooynta iyadoo loo eegayo qaabka qalabka tijaabada.
Codsiyada
ogaanshaha QPCR
Raridda iyo Kaydinta
Rarida:Xirmooyinka barafka
Xaaladaha Kaydinta:Ku kaydi at -20℃.
Shief Life:18 bilood
