Hal-tallaabo Multiplex RT-qPCR Premix
Sharaxaada
Bisadda No: HCR5141A
Multiplex One Tallaabo RT-qPCR Premix waa qalab PCR tiro badan oo ku salaysan RNA qaab ahaan.Tijaabada gudaheeda, qoraal-ku-noqosho iyo PCR tireed ayaa lagu sameeyaa isla tuubada falcelinta, fududaynta hawlgalka tijaabada ah iyo yaraynta khatarta wasakheynta.Naqshadaynta gaarka ah ee kaydinta iyo isku dhafka enzyme waxaa loo isticmaali karaa hal-tallaabo nidaamka lyophilized.Xirmada ayaa ka faa'iidaysata kuleyl u adkeysiga Reverse Transcriptase si ay si hufan u soo saarto cDNA xarriiqda kowaad iyadoo la isticmaalayo hotstart Taq DNA Polymerase si loo kordhiyo tirada.Waxay ka kooban tahay falcelinta la hagaajiyay, isku dhafka enzymes iwm, iyo arrimo si wax ku ool ah u joojiya kor-u-qaadista PCR-ka ee aan gaarka ahayn isla markaana kor u qaadaya waxtarka kordhinta fal-celinnada qPCR ee badan ayaa lagu daray, taasoo awood u siinaysa kor-u-qaadista tirada badan ee fluorescence iyadoo la hubinayo hufnaanta kor u qaadista aasaasiga ah.
Qaybaha
| Magaca |
| 1. Lyo-Buffer |
| 2. Isku darka lyo-enzyme |
| 3. ilaaliye Lyo |
Xaalada gaadiidkaion
J: Lyo-buffer iyo ilaaliye: -25~-15℃, nolosha shelf waa 1 sano.
B: Isku darka lyo-enzyme, 2-8 ℃, nolosha shelf waa 6 bilood.
Tilmaamaha hawlgalka
1. Nidaamka falcelinta (Tusaale ahaan u qaado 25μL)
| Qaybaha | Mugga (μL) | U-fiirsashada kama dambaysta ah |
| Lyo-Buffer | 6 | 1* |
| Isku-dhafka 'Lyo-Enzyme' | 1 | - |
| Lyo-Protectant | 8 | - |
| Isku dhafka koowaad (10μM) | 1 | 0.1-1uM |
| Isku-dhafka baaritaanka (10μM) | 0.5 | 0.05-0.5uM |
| RNA Template | 5 | - |
| DEPC H2O | Ilaa 25 | - |
2. Hab-maamuuska Baaskiilka oo la Habeeyay
1) Habka Baaskiilka caadiga ah
| Marxaladda falcelinta | Heerkulka | Waqtiga | Wareegto | |
| 1 | Dib u qorista | 50°Ca | 10min | 1 |
| 2 | Denaturation bilowga ah | 95°C | 5min | 1 |
| 3 | Falcelinta weynaynta | 95°C | 15 ilbiriqsi | 45 wareeg |
| 60°Cb | 30 ilbiriqsic |
2) Baratakoolka Baaskiilka Degdegga ah
|
| Marxaladda falcelinta | Heerkulka | Waqtiga | Wareegto |
| 1 | Dib u qorista | 50°Ca | 2 daqiiqo | 1 |
| 2 | Denaturation bilowga ah | 95°C | 2 ilbiriqsi | 1 |
| 3 | Falcelinta weynaynta | 95°C | 1 ilbiriqsi |
45 wareeg |
| 60°Cb | 13 ilbiriqsic |
Fiiro gaar ah:
a) Gadaal qoraal ahaan: Heerkulka wuxuu dooran karaa 42 ° C ama 50 ° C 10-15 daqiiqo.
b) Falcelinta weynaynta: Heerkulka waxaa lagu hagaajiyaa iyadoo loo eegayo qiimaha Tm ee aasaasayaasha nashqadeeyay.
c)Fluorescence calaamad helidFadlan u deji nidaamka tijaabada si waafaqsan shuruudahabuug-gacmeedka qalabka.
Macluumaadka Farsamada/Qeexitaannada
| Bilow kulul | Bilawga kulul ee la dhisay |
| Habka ogaanshaha | Ogaanshaha hore ee baaritaanka |
| Habka PCR | Hal tallaabo RT-qPCR |
| Nooca muunada | RNA |
Xusuusin
1. Alaabtan waxaa loogu talagalay isticmaalka cilmi-baarista oo keliya.
2. Fadlan xidho PPE-ga lagama maarmaanka ah, sida jaakada shaybaadhka iyo galoofyada, si loo hubiyo caafimaadkaaga iyo badbaadadaada!
