dATP/dCTP/dGTP/dTTP/dUTP (100mM)
Sharaxaada
Alaabtani waa xal dareere ah oo aan midab lahayn.Waxay ku habboon tahay tijaabooyinka bayooloojiyada molecular ee kala duwan sida kor-u-qaadista PCR, PCR-waqtiga-dhabta ah, cDNA ama isku-darka DNA-ga caadiga ah, taxanaha DNA iyo calaamadaynta.Waxaa lagu qasi karaa biyo saafi ah oo aad u saafi ah, waxaana lagu hagaajin karaa pH 7.0 oo leh saafi-sare NaOH, oo leh daahir ≥ 99% (HPLC).Marka la ogaado ka dib, kuma jiraan DNA-ga, RNase iyo phosphotase.Waxaa si toos ah loogu isticmaali karaa fal-celin bayooloji oo kala duwan sida PCR.
Qaab dhismeedka kiimikada
qeexid
| Walxaha tijaabi | Tilmaamaha |
| Muuqashada | Cad xal aan midab lahayn |
| Xoog-saarid | 100mM ± 3% |
| Daahirnimada (HPLC) | ≥99% |
| PH(22-25°C) | 7.0±0.1 |
| Xakamaynta tayada1.Diyaarintani waa ka nadiif wasakhda DNA-ga iyo RNase 2. Inta badan dNTP waxaa lagu tijaabiyay Taq DNA polymerase iyo Pfu DNA polymerase Intan d NTP waxay leedahay waxqabad lagu tijaabiyay Q-PCR | |
Codsiyada
PCR-dheer (20kb)
Isku-dhafka cDNA iyo RT-PCR
PCR-waqtiga dhabta ah
PCR caadiga ah
PCR kalsoonida sare
Raridda iyo Kaydinta
Gaadiidka:Xirmooyinka barafka
Xaaladaha Kaydinta:Ku kaydi at -25～-15℃
Taariikhda dib-u-imtixaanka lagu taliyay:2 sano
Xusuusin
1) Waxaa lagu milmi karaa heerkulka qolka.Kala diri ka dib, waa in lagu kaydiyaa sanduuqa barafka ama qubeyska barafka.Isticmaalka ka dib, waa in lagu kaydiyaa -25～-15 ℃ isla markiiba.
2) Badbaadadaada iyo caafimaadkaaga, fadlan xidho jaakadaha shaybaadhka iyo galoofyada la tuuri karo si loogu shaqeeyo.
