Poly A Carrier RNA
Bisadda No: HC4001A
Poly A, polyadenylate, waa isku dhafka 100 ~ 10000 polyadenylates, kaas oo lagu polymerized by fosforylase polynucleotide in vitro.In vivo, poly (a) waxaa lagu daraa 3-terminal ee mRNA enzyme si loo hagaajiyo xasiloonida mRNA.Codsiga soo saarista aashitada nucleic, ku darista poly A ee lysate ama xalinta xidhitaanka waxay hagaajin kartaa dhalidda DNA iyo RNA.Farsamayntaof poly A hagaajinta dhalidda nucleic acid waa sida soo socota:
1. Xidhiidh buuxa oo la xidhiidha xayaysiimaha korka ee maqaallada.Inta badan walxaha polypropylene waxay leeyihiin koronto joogto ah oogada, taas oo soo jiidan doonta asiidhyada nucleic.Side RNA ayaa buuxin kara kuwansaamaynta adsorption iyo yaraynta luminta asiidhyada nucleic-ka ah ee la beegsanayo.
2. Nucleases raad aan dhaq-dhaqaaq lahayn: Waxaa jira nukleases kala duwan oo ku jira muunadaha bayoolojiga iyodeegaanka.Poly A waxay dami kartaa xudunta raadadka ee tillaabooyinka soo saarista ama ilaalintahagaajinta wax-soo-saarka iyo xasilloonida asiidhyada nucleic-ka ah ee la beegsanayo.
3. Isku-duubni: Tallaabada nadiifinta aashitada nucleic-ka ee aalkolada dhexdhexaadinaysa roobab ama xidhidh, poly A waxay la jaanqaadi kartaa bartilmaameedka nucleic acid ama waxay samayn kartaa qaybo polymer sihagaajinta soo kabashada.
Xaaladaha kaydinta
-20 ~ 8 ℃, kaydinta qalalan, kaydinta muddada dheer waa in lagu dhejiyaa -20 ℃
Tilmaamid
| Lambarka CAS | 26763-19-9 |
| Muuqashada | Budada lyophilized cad |
| daahirnimo | 99% |
| Miisaanka Molecular | 700-3500 KDa |
Habka isticmaalka
Qaado cadad habboon oo budada lyophilized ah, ku dar biyo DEPC la daweeyay ama milixda guanidineku milmi 0.1-1ug/uL, ka dibna baakad hoose ku rid oo ku kaydi -20°C.
Codsiyada
1.Virus DNA / RNA soo saarista: ku darida 1-5ug Carrier RNA ee lysate waxay hagaajin kartaa wax soo saarka RNA / DNA, dejiso bartilmaameedka nucleic acid oo ka fogaato hoos u dhaca acid nucleic ee la safeeyey inta lagu jiro kaydinta.
2. Soo saarista micro DNA/RNA ee habka xuubka tiirka (<1ug), ku darida side RNA ilaa 1-5ug ayaa waxtar u leh si loo hagaajiyo dhalidda nucleic acid.
3. Dhexdhexaadinta aalkolada dhexdhexaadinta roobka aashitada nucleic iyo tallaabada fiirsashada, ku darida 1-2ug side RNA waxay caawinaysaa hagaajinta soo kabashada qaybta gaaban ee RNA.
4. Baaritaanka tirada PCR xalalka falcelinta, ku darida 10-100ng side RNA ee xalka falcelinta ayaa caawinaysa hagaajinta dareenka iyo yaraynta CTqiimaha.
