Uracil DNA Glycoylase (Glycerol-free)
Sharaxaada
Thermosensitive UDG (uracil-DNA glycosylase) waxay kicin kartaa hydrolysis ee saldhigga uracil ee silsiladda DNA ee ka kooban uracil iyo curaarta N-glycosidic ee laf-dhabarka sonkorta-phosphate si loo sii daayo uracil bilaash ah.Marka la barbar dhigo enzymes-ka caadiga ah ee UDG, UDG-da heerkulbeegga ah waxay ka fogaataa waxqabadka suurtogalka ah ee haraaga enzymes-ka caadiga ah ee UDG ka dib marka la hawlgeliyo, taas oo hoos u dhigi karta alaabada xoojinta dU-ka kooban heerkulka qolka.Alaabtani waxay ku shaqeysaa heerkulka qolka waana mid xasaasi u ah heerkulka oo u nugul firfircoonida.
Qaab-dhismeedka Kiimikada
Tilmaamid
| Enzyme | Glycosylase |
| Buffer ku habboon | Kaydinta kaydinta |
| Dhaqdhaqaaq la'aanta kulaylka | 50°C, 10 daqiiqo |
| Qeexitaan Unug | Hal unug (U) ayaa lagu qeexaa sida qadarka insaymka loo baahan yahay si uu u kiciyo hydrolysis ee 1 μg dU-ka kooban dsDNA gudaha 30 daqiiqo 25°C. |
Codsiyada
Ka saar du-ka ku jira PCR wasakhowga hawada hawada.
Ka-saarida saldhigyada uracil DNA-da hal-xadhigleed ama laba-geesood ah
Raridda iyo Kaydinta
Gaadiidka:Xirmooyinka barafka
Xaaladaha Kaydinta:Ku kaydi at -15 ℃ ~ -25 ℃
Nolosha Shief:1 sano
