Xirmada baaritaanka DNAase (Fluorescence)
Sharaxaada
Xirmada ogaanshaha DNA-da waxay ku saleysan tahay baaritaanka DNA-da ee fluorophore-ku sumadeeyey.Marka muunadku aanu ku jirin firfircoonida DNA-ga, baadhitaanku wuu xasiloon yahay mana soo saaro calaamada fluorescent;marka muunadku ka kooban yahay waxqabadka DNA-ga, baariddu waa hoos u dhacdaa, taasoo keenta calaamad si tartiib ah u kordheysa;heerka korodhka calaamada fluorescence waxay si togan ula xiriirtaa tirada iyo waxqabadka enzymes.Isticmaal akhristaha microplate fluorescence si aad u cabbirto hirarka dhererka ex/em=485/525nm si loo go'aamiyo in muunada ay wasakhowday DNA
Qaab-dhismeedka Kiimikada
Halabuurka Kit
| Magaca | 48Tijaabi | 192 Imtixaan |
| Xalka falcelinta 10× | 0.5ml | 2.0ml |
| baaritaanka DNA | 1 tube | 1 tube |
| TE baffer | 0.5ml | 2.0ml |
| Heerka DNAse I (2U/μL) | 10μL | 20μL |
| Halbeegga Dilution Buffer | 6ml | 12ml |
| DNAse & Biyo aan sanka lahayn | 25ml | 25ml |
| DNSase RNase ka fog | 50ml | 50ml |
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Muuqashada | Baakad dhammaystiran oo wax dareere ah ma daadin |
| Xadka ogaanshaha | DNA-ga I: 1.25x10-6U/μL |
| Saxnaanta | Intra assay CV ≤ 10% |
Gaadiidka iyo kaydinta
Gaadiidka:Baraf qallalan
Kaydinta:Ku kaydi heerkulka -25 ~ -15 ° C (iska ilaali qaboojinta soo noqnoqda iyo dhalaalidda)
Lagu taliyay in dib loo tijaabiyo Nolosha:1 sano
