Phosphatase Alkaline (ALP)

Phosphatase Alkaline waxaa laga soo qaatay dib-u-habayn E. koli ah oo sidda hidda-wadaha TAB5.Insaymku wuxuu kiciyaa dephosphorylation 5′ iyo 3′ darafyada DNA iyo fosphomonoester RNA.Sidoo kale, waxay hydrolyses ribose, iyo sidoo kale deoxyribonucleoside triphosphates (NTPs iyo dNTPs).TAB5 Phosphatase Alkaline wuxuu ku shaqeeyaa 5′ soo bixitaan, 5′ dib u dhac ah iyo dhammaad aan fiicneyn.Phosphatase-ka waxaa loo isticmaali karaa codsiyo badan oo bayoolaji molecular, sida calaamadaynta xinjirowga ama baaritaanka dhamaadka si meesha looga saaro darafyada fosfooraska ee DNA ama RNA.Tijaabooyinka cloning, dephosphorylation waxay ka hortagtaa DNA-da plasmid-ka ee toosan ee is-ligation-ka.Waxa kale oo ay hoos u dhigi kartaa dNTP-yada aan la wadaagin ee falcelinta PCR si loogu diyaariyo qaab-dhismeedka DNA-da.Insaymku si buuxda oo aan dib loo rogi karin ayaa loo hawlgeliyay kuleylinta 70°C ilaa 5 daqiiqo, taaso ka dhigaysa ka saarida fosfatada ka hor ligation-ka ama dhamaadka calaamadaynta mid aan loo baahnayn.