Xaddidaadda BspQI Endonuclease
Sharaxaada
BspQI waxa lagu soo celin karaa E. coli oo aqoonsan kara goobo gaar ah oo lagu soo saaray heerarka GMP.Waxaa si weyn loogu isticmaalaa khariidaynta hidda-wadaha, cloning iyo meelaha kale.Insaymiskani wuxuu si degdeg ah u gooyaa DNA-da si hufan oo toosan.
5'······ GGTCTC(N) 1·······3'
3'······CCAGAG(N)4·······5'
Qaab-dhismeedka Kiimikada
Qeexitaan Unug
Hal unug waxa lagu qeexaa cadadka insaymiska loo baahan yahay si loo dheefshiido 1 µg of λ DNA 1 saac 50°C ee mugga falcelinta guud ee 50 µl.
Tilmaamaha Alaabta
• Xannibaadda endonuclease waxay leedahay firfircooni enzymatic sare leh waxayna si dhakhso ah u dhamaystirtaa dheefshiidka.
• Dhaqdhaqaaqa endonuclease oo hooseeya oo aan gaar ahayn si loo hubiyo goynta saxda ah ee "scalpel".
• Ma jiro nidaam BSA, oo yareynaya wasakhowga isha kulaylka.
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Nadiifnimada borotiinka | 98% |
| Hadhaaga Protease | Midna lama ogaan |
| Isku xirka iyo goynta ogaanshaha | Waxtarka dheefshiidka;95% |
| DNA | Midna lama ogaan |
| Nicaknase | Midna lama ogaan |
Gaadiidka iyo kaydinta
Gaadiidka:Lagu soo raray wax ka yar 0 °C
Kaydinta:Ku kaydi heerkulka -20 °C
Lagu taliyay in dib loo tijaabiyo Nolosha:2 sano
