T4 RNA ligase
Sharaxaada
Alaabtani waa ligase ATP-ku-tiirsan T4 RNA (T4 RNA Ligase I) oo ay dib u soo bandhigtay E. coli.Enzyme Tani waxay kicisaa samaynta isku xidhka fosfodiester ee u dhexeeya oligonucleotides, RNA hal-xadhigle ah iyo DNA intermolecular / intra-molecular 5'-PO4 iyo 3'-OH.
Qeexitaan Unug
Hal unug waxa lagu qeexaa cadadka insaymka loo baahan yahay si loogu beddelo 1 nanomole of 5′-[³²P] rA16 oo loo beddelo foom u adkaysta fosfatase 30 daqiiqo 37°C.
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Dhaqdhaqaaqa Enzyme | 10 U/μL |
| Waxqabadka borotiinka | ≥ 95% |
| Nickase | Lama ogaan karo |
| Waxqabadka RNase | Lama ogaan karo |
| Ecoli gDNA | ≤1 koobi/50U |
| Endotoxin | Imtixaanka LCL, ≤10EU/mg |
Gaadiidka iyo kaydinta
Gaadiidka:Baraf qallalan
Kaydinta:Ku kaydi heerkulka -25 ~ -15 ° C (iska ilaali qaboojinta soo noqnoqda iyo dhalaalidda)
Lagu taliyay in dib loo tijaabiyo Nolosha:2 sano
alaabta la xidhiidha
Halkan ku qor fariintaada oo noo soo dir
