Bst 2.0 DNA Polymerase (Glycerol bilaash ah, cufnaan sare)
Bst DNA polymerase V2 waxaa laga soo qaatay Bacillus stearothermophilus DNA Polymerase I, kaas oo leh 5′→3′ firfircoonida polymerase DNA iyo dhaqdhaqaaqa beddelka silsiladda xooggan, laakiin ma jiro 5′→3′ waxqabadka exonuclease.Bst DNA Polymerase V2 waxay ku habboon tahay barokaca strand-ka, laambad-weynaynta isothermal (loop mediated isothermal amplification) iyo isku xigxig degdeg ah.Tani Bst DNA polymerase V2 waxay awood u leedahay in ay joojiso dhaqdhaqaaqa DNA polymerase heerkulka qolka, si loo shaqeeyo loona habeeyo habka falcelinta waxaa lagu samayn karaa heerkulka qolka, ka hortagga kor u qaadida aan gaar ahayn iyo hagaajinta waxtarka falcelinta, iyo noocaan ayaa kara lyophilized.Intaa waxaa dheer, waxay awood u leedahay inay ku sii deyso hawsheeda heerkul sarreeya, sidaas awgeed waxay tirtiraysaa baahida loo qabo tallaabo firfircooni gooni ah.
Qaybaha
| Qayb | HC5007A-01 | HC5007A-02 | HC5007A-03 |
| Bst DNA polymerase V2 (Glycerol-free) (32U/μL) | 0.05 ml | 0.25 ml | 2.5 ml |
| 10×HC Bst V2 Buffer | 1.5 ml | 2×1.5 ml | 3×10 ml |
| MgSO4 (100mM) | 1.5 ml | 2×1.5 ml | 2×10 ml |
Codsiyada
1.laambad-weynaynta isothermal
2.Xarun DNA oo falcelin barakac ah
3.Taxanaha hiddo-wadaha GC-ga sare
4.Isku xigxiga DNA ee heerka nanogram.
Xaalada Kaydinta
Gaadiidka ka hooseeya 0°C waxaana lagu kaydiyaa -25°C~-15°C.
Qeexitaan Unug
Hal unug ayaa lagu qeexaa cadadka ensaymka ee ku dara 25 nmol ee dNTP walax aan milmin aashitada 30 daqiiqo 65°C.
Falcelinta LAMP
| Qaybaha | 25 μLNidaamka |
| 10×HC Bst V2 Buffer | 2.5 μL |
| MgSO4 (100mM) | 1.5 μl |
| dNTPs (10mM midkiiba) | 3.5 μl |
| SYTO™ 16 Cagaar (25×)a | 1.0 μL |
| Isku dhafka koowaadb | 6 μL |
| Bst DNA Polymerase V2 (Glycerol-free) (32 U/ul) | 0.25 μl |
| Template | × μL |
| ddH₂O | Ilaa 25 μl |
Xusuusin:
1) ah.SYTOTM 16 Cagaaran (25×): Marka loo eego baahida tijaabada ah, midabyo kale ayaa loo isticmaali karaa beddelka;
2) b.Isku dhafka koowaad: waxaa lagu helay isku darka 20 µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB iyo qiyaaso kale.
Falcelinta iyo Xaaladda
1 × HC Bst V2 Buffer, heerkulku wuxuu u dhexeeyaa 60°C iyo 65°C.
Dhaqdhaqaaq la'aanta kulaylka
80°C, 20 daqiiqo.
