RT-LAMP Fluorescent (kubad lyophilized)
RT-LAMP Fluorescent Master Mix (Budada Lyophilized) waxay ka kooban tahay falcelinta falcelinta, polymerase Bst DNA iyo transcriptase u adkaysta kulaylka, difaaca lyophilized, iyo dheeha fluorescent.Alaabtu waxa ay ku jirtaa qaabka kuul la lyophilized, oo kaliya aasaasayaasha iyo qaab-dhismeedka ayaa loo baahan yahay in lagu daro isticmaalka.
Qayb Reagent
RT-LAMP Fluorescent Master Mix (Budada Lyophilized)
Isticmaalka
Si loo kordhiyo DNA-ga ama RNA isothermal
Xaaladaha Kaydinta
Gaadiidka iyo kaydka at 2 ~ 8°C.
Nolosha shelf: 12 bilood.
Baratakoolka
1. Ka soo saar tirada u dhiganta ee kuullaha lyophilized iyadoo loo eegayo tirada imtixaannada.
2. diyaarinta habka falcelinta
| Qayb | Qiyaasta |
| RT-LAMP Fluorescent Master Mix (Budada lyophilized) | 2 kuul |
| dheeha Floorescent | x μL |
| 10 × Asal ahaan Isku dhafka a | 5 μL |
| Qaababka DNA/RNA b | n μL |
| Wadarta | 50 μL |
Xusuusin:
1) ah.10×Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;
2) b.Qaabka aashitada nukliyeerka waxaa lagu talinayaa in lagu milmo biyaha DEPC.
Hal qayb oo ka mid ah Hyasen Biotechnology Co., Ltd
3) Barnaamijka falcelinta
| Heerkulka | Waqtiga | Wareegtada |
| 65℃ | 60-kii a | 30 |
Xusuusin:
1. Uruuri calaamadda fluorescent
Xusuusin
1. Heerkulka falcelinta waxa lagu hagaajin karaa inta u dhaxaysa 62°C iyo 68°C marka loo eego furahashuruudaha;
2. Tijaabadu waa in ay habeeyaan hawlgalka, oo ay ku jiraan diyaarinta habka falcelinta.habaynta muunada iyo qiyaasta;
3. Si looga fogaado faddaraynta, waxaa lagu talinayaa in lagu diyaariyo habka falcelinta miis aad u nadiif ahoo ku dar qaabyaalo daboolka qiiqa ee qolalka kale si looga fogaado faragelin been ah.
