D-Lactate dehydrogenase (LDH)
Sharaxaada
Enzyme waxaa loo isticmaalaa ka saarista pyruvate ee go'aaminta la shaqeynta NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Isticmaalka
1. Phosphatase alkaline oo lagu daray borotiinada (ka-hortagga unugyada, streptavidin iwm,) waxay si gaar ah u aqoonsan karaan molecules bartilmaameedka, waxaana loo isticmaali karaa ELISA, WB iyo ogaanshaha histochemical;
2.Alkaline fosphatase waxaa loo isticmaali karaa in lagu nadiifiyo 5 '-terminal DNA ama RNA si looga hortago isku-xirnaanta;
3.DNA ama RNA-ga kor ku xusan ee dephosphorylated waxa lagu calaamadin karaa fosfooraska raadiyaha ku suntan (iyada oo loo sii marayo T4 poly-nucleotide kinase)
Qaab-dhismeedka Kiimikada
Habka falcelinta
D-Lactate + NAD+→ Pyruvate +NADH + H+
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Sharaxaada | Budada amorphous cad, lyophilized |
| Hawsha | ≥200U/mg |
| Daahirnimada(SDS-BOG) | ≥90% |
| Solubility (10mg budada/ml) | Cad |
| NADH/NADPH oxidase | ≤0.01% |
| Malate fuuqbax | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Gaadiidka iyo kaydinta
Gaadiidka:Deegaanka
Kaydinta:Ku kaydi -20°C (Muddo dheer), 2-8°C (muddada gaaban)
Dib-u-imtixaan lagu taliyayNolosha:2 sano
