Phosphatase Alkaline (ALP)

Phosphatase Alkaline waxa laga soo qaatay dib-u-habayn E. koli ah oo sidda hidda-wadaha TAB5.Insaymku waxa uu kiciyaa dephosphorylation 5′ iyo 3′ darafyada DNA iyo RNA fosphomonoesters.Sidoo kale, waxay hydrolyses ribose, iyo sidoo kale deoxyribonucleoside triphosphates (NTPs iyo dNTPs).TAB5 Phosphatase Alkaline wuxuu ku shaqeeyaa 5′ soo bixitaan, 5′ dib u dhac ah iyo dhammaad ba'an.Phosphatase-ka waxaa loo isticmaali karaa codsiyo badan oo bayooloji molecular, sida calaamadaynta xinjirowga ama baaritaanka dhamaadka si meesha looga saaro darafyada fosforyaalka ah ee DNA ama RNA.Tijaabooyinka cloning, dephosphorylation waxay ka hortagtaa DNA-da plasmid-ka ee toosan ee is-ligation.Waxa kale oo ay hoos u dhigi kartaa dNTP-yada aan la wadaagin ee falcelinta PCR si loogu diyaariyo qaab-dhismeedka DNA-da.Insaymku si buuxda oo aan leexleexad lahayn ayaa loo hawlgeliyaa isagoo kuleyliya 70°C muddo 5 daqiiqo ah, taasoo ka dhigaysa ka saarida fosfatada ka hor inta aan la isku dhejin ama dhamaadka calaamadinta aan loo baahnayn.