Ribonuclease A (RNase A)

Ribonuclease A (RNase A) waa polypeptide-ka-xidhan oo ka kooban 4 curaarta disulfide oo leh miisaan kelli ah oo ku saabsan 13.7 kDa.RNase A waa endoribonuclease kaas oo si gaar ah hoos u dhigaya RNA hal-xadhigleed ee C iyo U hadhaaga.Gaar ahaan, kala-goynta waxay aqoonsanaysaa isku-xidhka fosfodiester-ka ee ay sameeyeen 5'-ribose ee nucleotide iyo kooxda fosfateetka ee 3'-ribose ee nucleotide pyrimidine ee ku xiga, si 2', 3' - fosfatiyeyaasha cyclic ay hydrolyzed si u dhigma. 3'-nucleoside phosphates (tusaale, pG-pG-pC-pA-pG waxaa kala jeexjeexay RNase A si loo dhaliyo pG-pG-pCp iyo A-PG).RNase A waa kan ugu firfircoon ee kala jeexjeexa hal-xadhigleed RNA wuxuuna ku firfircoon yahay xaalado falcelineed oo kala duwan: milixdu yar tahay (0 ilaa 100 mM NaCl), waxaa loo isticmaali karaa in lagu kala jeexo hal-xadhigle RNA, RNA labajibbaaran, iyo xargaha RNA ee isku-dhafka RNA-DNA.halka milixdu ay aad ugu badan tahay (≥0.3 M), RNase A waxay si gaar ah u jebin kartaa RNA hal-xadhig leh.