Glucose Dehydrogenase (GDH)
Sharaxaada
Glutamate Dehydrogenase (GDH) waa enzyme mitochondrial kaas oo kicinaya deamination oxidative ee glutamate ilaa a-ketoglutarate waxayna u adeegtaa sidii isku xirka muhiimka ah ee u dhexeeya waddooyinka anabolic iyo catabolic.Naasleyda, GDH waxay ku xiran tahay nidaaminta allosteric waxayna leedahay dhaqdhaqaaq sare oo beerka, kelyaha, maskaxda, iyo beeryarada.Dhaqdhaqaaqa GDH ee serum waxaa loo isticmaali karaa in lagu kala saaro cudurrada beerka sababtoo ah caabuqa beerka, taas oo aan muujinaynin dhaqdhaqaaqa sare ee serum GDH, iyo cudurrada keena hepatocyte necrosis, taas oo keenta serum GDH oo sarreeya.
Dhaqdhaqaaqa GDH waxaa lagu go'aamiyaa baaritaanka enzyme la isku daray kaas oo glutamate uu cunay GDH soo saarta NADH, kaas oo ka falceliya baaritaanka soo saaraya badeecad midab leh (450 nm) oo u dhiganta hawsha GDH ee jirta.Hal unug oo GDH ah waa qadarka enzyme ka soo saari doona 1.0 mmle NADH daqiiqadii pH 7.6 at 37 °C
Qaab-dhismeedka Kiimikada
Habka falcelinta
D-glucose + aqbalaha → D-Glucono-1,5-lactone + aqbalaha oo yaraada
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Sharaxaada | Budada amorphous cad, lyophilized |
| Hawsha | ≥160U/mg |
| Daahirnimada(SDS-BOG) | ≥90% |
| Solubility (10 mg budada/ml) | Cad |
| Ensaymes wasakhaysan | |
| Glucose dehydrogenase (NAD) | ≤0.02% |
| Hexokinase | ≤0.02% |
| A-Glucosidase | ≤0.02% |
Gaadiidka iyo kaydinta
Gaadiidka: Xirmooyinka barafka
Kaydinta:Ku kaydi heerkulka -25 ~ -15 ° C (mudda dheer), 2-8 ° C (muddada gaaban)
Dib-u-imtixaan lagu taliyayNolosha: 18 bilood
