Peroxidase (Isha Horseradish) La mid ah: Hydrogen peroxide oxidoreductase;HRP

Horseradish peroxidase (HRP) waxay ka go'day xididdada malayga (Amoracia rusticana) waxayna ka tirsan tahay kooxda ferroprotopoorphyrin ee peroxidases.HRP waxay si sahal ah ugu biirtaa hydrogen peroxide (H2O2).Natiijooyinka [HRP-H2O2] isku-dhafan ayaa ogaysiinaya noocyo badan oo deeq-bixiyeyaasha hydrogen ah:
Ku-deeqaha + H2O2 → Ku-deeqaha Oxidized + 2 H2O
HRP waxay oksidhiyaysaa substrates kala duwan (eeg Shaxda 1):
• Chromogenic
• Chemiluminescent (sida luminol ama isoluminol)
• Fluorogenic (sida tyramine, homovanillic acid, ama 4-hydroxyphenyl acetic acid)
HRP waa hal silsilad polypeptide ka kooban afar buundooyinka disulfide.HRP waa glycoprotein ka kooban 18% karbohaydrayt.Halabuurka karbohaydraytku wuxuu ka kooban yahay galactose, arabinose, xylose, fucose, mannose, mannosamine, iyo galactosamine, taas oo ku xidhan isozyme gaar ah.

HRP waa calaamad si weyn loo isticmaalo oo loogu talagalay immunoglobulins ee codsiyada immunochemistry kala duwan, oo ay ku jiraan immunoblotting, immunohistochemistry, iyo ELISA.HRP waxa lagu xidhi karaa unugyada difaaca dhawr habab oo kala duwan, oo ay ku jiraan glutaraldehyde, periodate oxidation, iyada oo loo marayo curaarta disulfide, iyo sidoo kale amino iyo thiol-ku-gudbinta iskutallaabta.HRP waa calaamadda ugu badan ee loogu talagalay unugyada difaaca jirka, maadaama ay tahay tan ugu yar uguna xasilloon saddexda calaamadood ee ugu caansan enzyme (peroxidase, β-galactosidase, fosfatase alkaline) iyo glycosylation-keedu waxay keenaysaa in ay hoos u dhigto isku-xidhka aan gaarka ahayn.Dib u eegis lagu sameeyay glutaraldehyde iyo hababka isku xidhka xilliyada ayaa la daabacay.

Peroxidase sidoo kale waxaa loo isticmaalaa go'aaminta gulukooska4 iyo peroxides ee xalka.Dhowr daabacaad, 6-24 taxanayaal, 25-29 iyo qoraallo 30-46 ah ayaa soo xigtay isticmaalka P8375 borotokoolkooda cilmi-baarista.