Fructosyl-peptide Oxidase (FPOX)
Sharaxaada
Insaymku wuxuu faa'iido u leeyahay go'aaminta fructosyl-peptide iyo fructosyl-L-amino acid.
Qaab-dhismeedka Kiimikada
Mabda'a Falcelinta
Fructosyl-peptide + H2O + O2→ Peptide + Glucosone + H2O2
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Sharaxaada | Budada amorphous cad, lyophilized |
| Hawsha | ≥4U/mg |
| Daahirnimada(SDS-BOG) | ≥90% |
| Catalase | ≤0.01% |
| ATPase | ≤0.005% |
| Glucose oxidase | ≤0.03% |
| Kolestarool oxidase | ≤0.003% |
Gaadiidka iyo kaydinta
Gaadiidka: Ambient
Kaydinta:Ku kaydi -20°C (Muddo dheer), 2-8°C (muddada gaaban)
Dib-u-imtixaan lagu taliyayNolosha:2 sano
Taariikhda horumarka
Mid ka mid ah tusmooyinka loo isticmaalo ogaanshaha cudurka macaanka waa haemoglobin glycated (HbA1c).Cabbirka HbA1c iyadoo la isticmaalayo enzymes waxay ku habboon tahay in lagu farsameeyo tiro badan oo muunado ah, waana mid kharash badan.Sidan oo kale, waxaa jiray muddo dheer baaq xooggan oo ka yimid dhakhaatiirta caafimaadka ee horumarinta baaritaanka enzyme sida.Sidaa darteed, waxaanu samaynay imtixaan cusub anagoo adeegsanayna "habka dipeptide".Gaar ahaan, waxaan ogaanay "Fructosyl-peptide Oxidase" (FPOX) oo loo isticmaali karo enzym ahaan baaritaankan.Tani waxay fududaysay guushayada ah inaan gaarno aduunka ugu horeeya anagoo samaynay xaqiiqo ah HbA1c enzyme assay.Habkan "dipeptide" wuxuu isticmaalaa Protease (Proteolytic enzyme) si uu u burburiyo HbA1c ee dhiigga, ka dibna cabbiro heerarka dipeptides saccharified ee la soo saaro iyadoo la isticmaalayo FPOX.Habkani waxa uu la kulmay soo dhawayn aad u wanaagsan taas oo ay ugu wacan tahay mudnaantiisa in uu fudud yahay, raqiis ah oo degdeg ah, iyo reagent HbA1c ee la isticmaalayo FPOX ayaa hadda noqday in laga isticmaalo adduunka oo dhan.
