Hexokinase (HK)
Sharaxaada
U isticmaal Hexokinase go'aaminta D-glucose, D-fructose, iyo D-sorbitol ee cuntada ama muunadaha cilmi-baarista noolaha.Enzyme sidoo kale waxaa loo isticmaalaa baaritaanka saccharides kale kuwaas oo loo bedeli karo gulukoos ama fructose, oo sidaas awgeed faa'iido u leh baaritaanka glycosides badan.
Haddii Hexokinase loo isticmaalo in lagu daro glucose-6-fosfate dehydrogenase (G6P-DH)* (qiimaynta glucose6-phosphate ay samaysay Hexokinase), muunado waa in aanay noqon mid ka mid ah uruurinta fosfooraska sare sida G6P-DH waxaa si tartan ah u xannibay fosfattu.
Qaab-dhismeedka Kiimikada
Mabda'a Falcelinta
D-Hexose + ATP --mg2+→ D-Hexose-6-phosphate + ADP
Tilmaamid
Walxaha Tijaabada | Tilmaamaha |
Sharaxaada | Cadaan ilaa huruud yar budada amorphous, lyophilized |
Hawsha | ≥30U/mg |
Daahirnimada(SDS-BOG) | ≥90% |
Solubility (10mg budo/ml) | Cad |
borotiinka | ≤0.01% |
ATPase | ≤0.03% |
Fosfooglucose isomerase | ≤0.001% |
Creatine phosphokinase | ≤0.001% |
Glucose-6-Fosfat dehydrogenase | ≤0.01% |
NADH/NADPH oxidase | ≤0.01% |
Gaadiidka iyo kaydinta
Gaadiidka: Adeegaanka
Kaydinta:Ku kaydi heerkulka -20°C (mudda dheer), 2-8°C (muddada gaaban)
Dib-u-imtixaan lagu taliyayNolosha:1 sano