Kolestarool oxidase (COD/CHOD)
Sharaxaada
Kolesteroolka oxidase (CHOD) wuxuu kiciyaa tallaabada ugu horreysa ee catabolismka kolestaroolka.Qaar ka mid ah bakteeriyada aan bakteeriyada ahayn, sida Streptomyces ayaa awood u leh inay ka faa'iidaystaan kolestaroolka ilaha kaarboonka.Bakteeriyada cudur-sidaha leh, sida Rhodococcus equi, waxay u baahan tahay CHOD si ay u qaadsiiso makrophage-ka martida loo yahay.CHOD waa laba-shaqaale. Kolestaroolka ayaa markii hore oksaydhiyay kolestarool-5-en-3-hal tallaabo FAD u baahan.Cholest-5-en-3-one waa la soocay ilaa cholest-4-en3-one.Hawsha CHOD waxay ku xidhan tahay sifooyinka jidheed ee xuubka uu substrate-ku ku xidhan yahay.
CHOD waxaa loo isticmaalaa in lagu go'aamiyo kolestaroolka serum.Waa enzyme labaad ee loogu isticmaalka badan yahay codsiyada ogaanshaha ka dib glucose oxidase.CHOD waxa kale oo ay ka heleysaa arji ku jirta falanqaynta microanalysis ee steroids ee muunadaha cuntada iyo kala soocida 3-ketosteroids ee 3b-hydroxysteroids.Kolestarool oxidase ayaa sidoo kale loo isticmaalay sidii baaritaan molecular si loo caddeeyo qaababka xuubabka gacanta.
Qaab-dhismeedka Kiimikada
Mabda'a Falcelinta
Kolestaroolka + O2 →△4-Cholesten-3-hal + H2O2
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Sharaxaada | budada amorphous jaalaha ah, lyophilized |
| Hawsha | ≥8U/mg |
| Daahirnimada(SDS-BOG) | ≥90% |
| Solubility (10mg budo/ml) | Cad |
| Catalase | ≤0.001% |
| Glucose oxidase | ≤0.01% |
| Cholesterol sterase | ≤0.01% |
| ATPase | ≤0.005% |
Gaadiidka iyo kaydinta
Gaadiidka:Raray ka hooseeya -15 ° C
Kaydinta:Ku kaydi heerkulka -25 ~ -15°C (Muddo dheer), 2-8°C
Dib-u-imtixaan lagu taliyayNolosha:1 sano
