DNAse I (Rnase Bilaash) (2u/ul)
Bisadda No: HC4007B
DNAse I waa endonuclease kaas oo dheefi kara DNA-da hal-ama laba-geesooban.Waxay hydrolyzize kartaa bonds phosphodiester si ay u soo saarto mono-iyo oligodeoxynucleotides ka kooban 5'-fosfate group iyo 3'-OH group.Qiyaasta pH ee ugu fiican ee DNAse I waa 7-8.Hawlaha DNAse I waxay kuxirantahay Ca2+waxaana lagu hawlgelin karaa ion birta kala duwan sida CO2, Mn2+, Zn2+iwm. Iyadoo uu joogo Mg2+, DNA-da waxaan si aan kala sooc lahayn u kala goyn karaa goob kasta oo DNA-da laba-geesooban;Halka uu Mn2+, DNase Waxaan kala jebin karaa DNA-da laba-geesoon ee isla goobtaas, samaynta cidhifyo cad ama cidhifyo dhegdheg leh oo leh 1-2 nucleotide oo soo baxay.Waxaa loo isticmaali karaa habaynta shaybaarada RNA ee kala duwan.
Qaybaha
| Magaca | 1KU | 5KU |
| Dib-u-habaynta DNSaseI (RNase-free) | 500 μL | 5 × 500 μL |
| DNase I Reaction Buffer (10×) | 1 ml | 5 × 1ml |
Xaaladaha kaydinta
Alaabtan waa in lagu kaydiyaa at -25 ~ -15 ℃ 2 sano.Fadlan iska ilaali qaboojinta soo noqnoqda.
Tilmaamaha
Codsaday in laga saaro DNA-da shaybaarada RNA ee tixraaca kaliya.
1. Fadlan isticmaal tubbada centrifuge-free-RNase iyo talooyinka pipette si aad u diyaarisid habka falcelinta soo socota:
| Qaybaha | Mugga (μL) |
| DNase I Reaction Buffer (10×) | 1 |
| Dib-u-habaynta DNasel (RNase-free) | 1 |
| RNA | X |
| RNase-free ddH2O | Ilaa 10 |
2. Xaaladaha falcelintu waa sida soo socota: 37 ℃, 15-30 mins ka dib, ku dar fiirsashada kama dambaysta ah ee 2.5 mM EDTA oo si fiican u walaaq, ka dibna 65 ℃ 10 mins.Qaabka la farsameeyay waxa loo isticmaali karaa kuwa xiga ee RT-PCR ama tijaabooyinka RT-qPCR, iwm.
Xusuusin
1.DNase l waa u nugul denaturation jirka;Markaad isku qaseyso, si tartiib ah u rogo tuubada baaritaanka oosi fiican u rux, si xoog leh ha u ruxin.
2. Insaymiska waa in lagu kaydiyaa sanduuqa barafka ama qubeyska barafka marka la isticmaalo, waana in lagu kaydiyaa at -20 ℃ isla markiiba ka dib isticmaalka.
3. Alaabtan waxaa loogu talagalay isticmaalka cilmi-baarista oo keliya.
4. Fadlan ku shaqee jaakadaha shaybaadhka iyo galoofyada la tuuri karo, badbaadadaada awgeed.
