Dib-u-habaynta Deoxyribonuclease I (DNase I, RNase-free)
Sharaxaada
Dib-u-habaynta Deoxyribonuclease I, waa endonuclease kaas oo dheefshii kara DNA-da hal-xadhig leh ama laba-geesood ah.Waxay hydrolyzes-ku-xidhka phosphodiester-ka si ay u soo saarto mono- iyo oligodeoxynucleotides oo ka kooban 5'-fosfate kooxaha iyo 3'-OH kooxaha.Qiyaasta pH ee ugu fiican ee DNAse I waa 7-8.Dhaqdhaqaaqa DNase I waxay kuxirantahay Ca2 + waxaana lagu kicin karaa ion birta kala duwan sida Co2+, Mn2+, Zn2+, iwmhalka joogitaanka Mn2+, DNase Waxaan gooyn karaa DNA-da laba-geesoon ee isla goobta, samaynta cidhifyo aan fiicneyn ama 1-2 nuclei Sticky waxay ku dhammaataa nucleotide dushooda.Insaymiskan waxa uu ka yimid noocyada dib-u-dhaqaajinta E. coli, kuma jiraan RNase, waxaana loo isticmaali karaa habaynta muunado RNA oo kala duwan.
Qaab dhismeedka kiimikada
Codsiyada
Soo saarista RNA: diyaari RNA aan lahayn DNA;
Ka dib markii in vitro transcription la RNA polymerase, sida T7 RNA Polymerase (Cat # 10618), waxaa loo isticmaalaa in meesha laga saaro template DNA;
U diyaari RNA-free DNA ka hor RT-PCR iyo RT-qPCR;
Loo adeegsaday iyadoo lala kaashanayo DNA Polymerase I (Bisad #12903) si loogu calaamadiyo DNA-da tarjumaada nick;
Loo isticmaalo falanqaynta raad-raaca si loo falanqeeyo isdhexgalka DNA-protein;
Samee maktabad aan kala sooc lahayn;
Helitaanka TUNEL ee apoptosis, DNA-da genomic ee qayb ahaan la xiiray ayaa loo adeegsaday xakamayn togan.
Raridda iyo Kaydinta
Gaadiidka:Xirmooyinka barafka
Xaaladaha Kaydinta:Ku kaydi -20 ℃
Nolosha shelf2 sano
Xusuusin:
1.Enzymes waa in lagu kaydiyaa sanduuqa barafka ama qubeyska barafka marka la isticmaalo, waana in lagu kaydiyaa heerkulka -20°C isla markaaba isticmaalka ka dib.
2. Badbaadadaada iyo caafimaadkaaga, fadlan xidho qalabka ilaalinta shakhsi ahaaneed (PPE), sida jaakadaha shaybaadhka iyo galoofyada la tuuri karo, markaad alaabtan ku shaqaynayso.
