Hal tilaabo oo degdeg ah RT-qPCR Probe Premix-UNG
Bisadda No: HCR5143A
Hal Tilaabo RT-qPCR Kit Kit (FOR FAST) waa xirmada ogaanshaha degdega ah ee RT-qPCR ku salaysan oo ku habboon PCR hal-plex ama multiplex iyadoo la isticmaalayo RNA qaab ahaan (sida fayraska RNA).Alaabtani waxay isticmaashaa jiil cusub oo ka hortag ah oo la beddelay Taq DNA Polymerase iyo hal-tallaabo u go'ay Reverse Transcriptase, oo leh kayd la habeeyay ee kor u qaadis degdeg ah, kaas oo leh xawaare weynayn degdeg ah, hufnaan sare u qaadis iyo gaar ahaaneed.Waxay taageertaa kordhinta dheellitiran ee labadaba hal-plex iyo multix ee shaybaarrada fiirsashada hooseeya iyo kuwa sarreeya labadaba waqti gaaban gudaheed.
Qaybaha
1.5×RT-qPCR kaydiye (U+)
2. Isku darka Enzyme (U+)
Xusuusin:
a.5×RT-qPCR wax kaydiya (U+) waxa ku jira dNTP iyo Mg2+;
b.Isku darka Enzyme (U+) waxaa ka mid ah transcriptase gadaale, Hot Start Taq DNA polymerase, RNase inhibitor iyo UDG;
c.Isticmaal talooyinka RNase-free, tuubooyinka EP, iwm.
Kahor intaan la isticmaalin, si fiican u qas 5×RT-qPCR kaydiyaha (U+).Haddii ay jiraan wax roobab ah dhalaalidda ka dib, sug inta kaydku ku soo laabanayo heerkulka qolka, qas oo milaan, ka dibna si caadi ah u isticmaal.
Xaaladaha Kaydinta
Alaabta waxaa lagu raray baraf qallalan waxaana lagu kaydin karaa -25 ~ -15℃ 1 sano.
Tilmaamaha
1. Falcelinta Nidaamka
| Qaybaha | Mugga (20 μL falcelin) |
| 2 ×RT-qPCR kaydiye | 4μL |
| Isku darka Enzyme (U+) | 0.8μL |
| Horay u socod | 0.1 ~ 1.0μM |
| Dib-u-celinta hore | 0.1 ~ 1.0μM |
| TaqMan Probe | 0.05 ~ 0.25μM |
| Template | X μL |
| RNase-Biyo Xor ah | ilaa 25 μl |
Xusuusin: Mugga falcelinta waa 10-50μL.
2. Habka Baaskiilka (Standard)
| Wareegto tallaabo | Heerkulka | Waqtiga | Wareegtada |
| Dib u qorista | 55 ℃ | 10 daqiiqo | 1 |
| Denaturation bilowga ah | 95 ℃ | 30 ilbiriqsi | 1 |
| Denaturation | 95 ℃ | 10 ilbiriqsi | 45 |
| Baabi'inta/kordhinta | 60 ℃ | 30 ilbiriqsi |
| Habka Baaskiilka (Dhakhso) Wareegto tallaabo |
Heerkulka |
Waqtiga |
Wareegtada |
| Dib u qorista | 55 ℃ | 5 daqiiqo | 1 |
| Denaturation bilowga ah | 95 ℃ | 5 s | 1 |
| Denaturation | 95 ℃ | 3 s | 43 |
| Baabi'inta/kordhinta | 60 ℃ | 10 s |
Xusuusin:
a.Heerkulka qoraalka dib u celinta wuxuu u dhexeeyaa 50 ℃ ilaa 60 ℃, kordhinta heerkulku waxay gacan ka geysataa kor u qaadida qaabdhismeedka adag iyo qaababka sare ee CG;
b.Heerkulka ugu fiicnaanta ayaa loo baahan yahay in lagu hagaajiyo iyadoo lagu salaynayo qiimaha Tm ee furaha, oo dooro wakhtiga ugu gaaban ee ururinta calaamadaha fluorescence iyadoo lagu salaynayo qalabka PCR ee wakhtiga dhabta ah.
Xusuusin
Fadlan xidho PPE-ga lagama maarmaanka ah, sida jaakada shaybaadhka iyo galoofyada, si loo hubiyo caafimaadkaaga iyo badbaadadaada!
