Ribonuclease III (RNase III)
Sharaxaada
Alaabtani waa ribonuclease III (RNase III) oo ay dib u soo bandhigtay E. coli.Exonuclease-kan gaarka ah wuxuu kala jeexayaa RNA labajibbaaran (dsRNA) wuxuuna soo saaraa 12-35bp dsRNA jajabyo leh 5'-PO4 iyo 3'-OH, 3'
Qaab-dhismeedka Kiimikada
Qeexitaan Unug
Qeexitaannada unugga hawlqabadka: Hal unug waxa loola jeedaa qaddarka insaymiska loo baahan yahay si loo dhimo 1 μg of
dsRNA ilaa siRNA gudaha nidaamka falcelinta 50 μL at 37°C ilaa 20 daqiiqo.
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Waxqabadka Exonuclease | Waxay siidaayaan 0.1% wadarta shucaaca |
| Dhaqdhaqaaqa DNA-ga Aan Gaarka ahayn | Lama ogaan karo |
| Qiimaynta Nadiifinta Barootiinka (SDS-PAGE) | ≥ 95% |
| Waxqabadka RNase ( Dheefshiid Dheeraad ah) | 90% substrate RNA ayaa weli ah mid sugan |
Gaadiidka iyo kaydinta
Gaadiidka:Baraf qallalan
Kaydinta:Ku kaydi heerkulka -25 ~ -15°C
Lagu taliyay in dib loo tijaabiyo Nolosha:2 sano
alaabta la xidhiidha
Halkan ku qor fariintaada oo noo soo dir
