Wax-soo-saarka sare ee T7 RNA Poymerase
Sharaxaada
T7 RNA Polymerase waxay ka go'day E.coli oo sidata plasmid ka kooban T7 RNA Polymerase hidda-wadaha.T7 RNA Polymerase waa DNA-ku-tiirsan RNA polymerase kaas oo aad u gaar ah dhiirrigeliyeyaasha T7 phage.
Qaab-dhismeedka Kiimikada
Qeexitaan Unug
Hal unug ayaa lagu qeexaa cadadka ensaymka oo ku dari doona 1 nmol ATP walxo aashito- milmi kara wadarta guud ee mugga falcelinta ee 50 μl 1 saac 37°C gudaha 1X RNA Polymerase Reaction Buffer.
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Nadiifnimada borotiinka | ≥95% |
| Nickase | Midna lama ogaan |
| Rnase | Midna lama ogaan |
| Endonuclease | Midna lama ogaan |
| Exonuclease | Midna lama ogaan |
| Gaar ahaan dhiirigeliyaha | Alaab aan gaar ahayn≤1.5% |
| E.coli gDNA | ≤0.1 pg/50U |
| Endotoxin | Imtixaanka LAL, ≤10EU/mg |
Gaadiidka iyo kaydinta
Gaadiidka:Baraf qallalan
Kaydinta:Dhammaan qaybaha waa in lagu kaydiyaa heerkulka -25 ~ -15 ° C
Lagu taliyay in dib loo tijaabiyo Nolosha:2 sano
alaabta la xidhiidha
Halkan ku qor fariintaada oo noo soo dir
