Qalabka Qiimaynta Nukliyeerka ee Ultra (ELISA)
Sharaxaada
Xirmadan Ultra Nuclease ELISA waa ciid ELISA ah oo lagu sameeyo qaab microplate ah.Tusaalaha laga yaabo inay ku jiraan endonuclease ayaa lagu beeray ceelal saxan microtiter ah oo horay loogu dahaadhay xidhid la safeeyey anti-endonuclease antibody.Ka dib ka-soo-baxa iyo tillaabada dhaqidda oo laga saaro qaybaha aan xidhnayn, enzyme-conjugated, anti-endonuclease detector antibody ayaa lagu daraa.Dareen-celinta difaaca jirka waxay keentaa samaynta isku-dhafka sandwich ee wajiga adag ee antibody-endonuclease-enzyme oo ku suntan antibody.Ka dib markii tallaabada ugu dambeysa ee dhaqidda, xal substrate ah ayaa lagu daraa ceelasha iyo falcelinta, taasoo keentay horumarinta midabka.Cufnaanta indhaha waxaa lagu cabbiraa sawir-qaade ahaan waxayna la siman tahay kan
fiirsashada falanqeeye jooga ceelasha.U fiirsashada endonuclease ee muunado aan la garanayn ayaa lagu xisaabin karaa iyadoo lagu salaynayo qalooca heerka u dhigma.
Qaab-dhismeedka Kiimikada
Qeexitaan Unug
Qadarka enzyme loo isticmaalo in lagu beddelo qiimaha nuugista △A260 1.0 gudaha 30min
at 37 °C, pH 8.0, oo u dhiganta 37μg salmon oo la shiiday shahwada DNA iyadoo la jarayo oligonucleotides, ayaa lagu qeexay inay tahay unug firfircoon.
Isticmaalka iyo qiyaasta
• Ka saar aashitada nucleic-ka-baxsan ee alaabta tallaalka, yareyso halista haraaga sunta nucleic-ka iyo hagaajinta badbaadada alaabta.
• Yaree viscosity ee dareeraha quudinta ee ay keento nucleic acid, yarayso wakhtiga habaynta iyo kordhiso dhalidda borotiinka.
• Ka saar aashitada nucleic-ka ah ee ku duudduubtay qayb (fayras, jidhka ku darida, iwm.), kaas oo ku habboon sii daynta iyo sifaynta qaybta.
• Daawaynta Nukliyeerku waxay wanaajin kartaa xallinta iyo soo kabashada muunadda ee chromatography tiirka, electrophoresis iyo falanqaynta xoqidda.
• Daawaynta hidda-wadaha, aashitada nucleic ayaa laga saaraa si loo helo fayrasyo la safeeyey adeno oo la safeeyey.
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Xadka hoose ee ogaanshaha | 0.6 ng/ml |
| Xadka hoose ee tirada | 0.2ng/ml |
| Saxnaanta | Baaritaanka gudaha CV≤10% |
Gaadiidka iyo kaydinta
Gaadiidka:Lagu soo raray wax ka yar 0 °C
Kaydinta:Ku kaydi heerkulka -2-8°C, reageent furan oo xasiloon ilaa 6 toddobaad
Lagu taliyay in dib loo tijaabiyo Nolosha:1 sano
