Deoxyribonuclease I (Dnase I)
Sharaxaada
DNase I (Deoxyribonuclease I) waa endodeoxyribonuclease kaas oo dheefshiidka kara hal-ama laba-geesood DNA.Waxay aqoonsataa oo kala jeexdaa xidhmooyinka fosfodiester-ka si ay u soo saarto monodeoxynucleotides ama oligodeoxynucleotides hal-ama laba-laaban leh oo leh kooxaha fosfateetka ee 5'-terminal iyo hydroxyl ee 3'-terminal.Hawlaha DNAse I waxay ku xiran tahay Ca 2+ waxaana lagu kicin karaa ion birta kala duwan sida Mn 2+ iyo Zn 2+ .5 mM Ca 2+ waxay ka ilaalisaa enzyme-ka hydrolysis.Joogitaanka Mg 2+, ensaymku wuxuu si aan kala sooc lahayn u aqoonsan karaa oo u kala goyn karaa meel kasta oo ku taal qayb kasta oo DNA ah.Joogitaanka Mn 2+ , labada qaybood ee DNA-da ayaa isku mar la aqoonsan karaa oo la kala gooyn karaa ku dhawaad isla goobta si ay u sameeyaan jajabyo DNA ah oo siman ama jajabyo DNA ah oo dhegdheg leh oo leh 1-2 nucleotides.
Qaab-dhismeedka Kiimikada
Qeexitaan Unug
Hal unug waxa lagu qeexaa cadadka insaymka kaas oo si buuxda hoos ugu dhigi doona 1 μg ee pBR322 DNA 10 daqiiqo gudahood 37°C.
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| daahirnimo (SDS-BOG) | ≥ 95% |
| Waxqabadka Rnase | Hoos-u-dhac ma jiro |
| Wasakhaynta gDNA | ≤ 1 koobi/μL |
Gaadiidka iyo kaydinta
Gaadiidka:Lagu soo raray wax ka yar 0 °C
Kaydinta:Ku kaydi heerkulka -25 ~ -15°C
Lagu taliyay in dib loo tijaabiyo Nolosha:2 sano
