T7 RNA Poymerase (fiirsashada sare)
Sharaxaada
T7 RNA Polymerase waa DNA-ku-tiirsan RNA-olymerase ka T7 phage, kaas oo leh xoog leh oo gaar ah 5 ′→ 3 ′ RNA polymerase dhaqdhaqaaqa.T7 RNA Poplymerase waxay si gaar ah u leedahay taxanaha T7 waxayna ka soo saari doontaa tiro badan oo RNA ah jajab DNA ah oo la geliyo hoosta ka soo jeediyaha.
Qaab-dhismeedka Kiimikada
Qeexitaan Unug
Hal unug waxa lagu qeexaa cadadka insaymiska looga baahan yahay in lagu daro 1 nmol ATP shay aan milmi karin 1 saac gudahood 37°C
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Nadiifnimada borotiinka | ≥95% |
| Nickase | Midna lama ogaan |
| Rnase | Midna lama ogaan |
| Endonuclease | Midna lama ogaan |
| Exonuclease | Midna lama ogaan |
| Gaar ahaan dhiirigeliyaha | Lama ogaan karo alaabta aan gaarka ahayn |
| E.coli gDNA | ≤0.1 pg/50U |
| Endotoxin | Imtixaanka LAL, ≤10EU/mg |
Gaadiidka iyo kaydinta
Gaadiidka:ka hooseeya 0 °C
Kaydinta:Dhammaan qaybaha waa in lagu kaydiyaa heerkulka -25 ~ -15 ° C
Lagu taliyay in dib loo tijaabiyo Nolosha:2 sano
alaabta la xidhiidha
Halkan ku qor fariintaada oo noo soo dir
