AMPPD-Chemiluminescent Substrate
Sharaxaada
AMPPD waxay leedahay laba qaybood oo muhiim ah qaab dhismeedka molecular.Mid ka mid ah waa dioxygen
giraanta tetrad ee isku xidha giraanta benzene iyo adamantane, kaas oo jabin kara soona saari kara photons.Midda kale waa joogitaanka fosfatase alkaline, kaas oo kicinaya ka saarista kooxaha fosfatka waxayna sameeyaan dhexdhexaad aan degganayn.Dhexdhexaadku markaa si kedis ah ayuu u burburay oo u sii daayay sawiro, tirada photons ee la sii daayay waxay u dhigantaa fiirsashada ALP ee xalka, taas oo ku habboon nidaamka difaaca jirka ee chemiluminescence oo leh ALP oo ah ensaymka calaamadeysan.
Isticmaal:
AMPPD waa dareere chemiluminescent ah oo leh dareen sare, xasillooni iyo saamiga calaamad-ilaa-sanqa, kaas oo loo isticmaali karo difaaca jirka ee Phosphatase Alkaline (ALP) calaamad ahaan.
Qaab-dhismeedka Kiimikada
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Sharaxaada | Dareeraha cad ee cagaarka jaalaha ah |
| Xasilooni | Qiimaha Iuminescence ee 37℃ ayaa lagu hayaa in ka badan 90% ka dib 7 maalmood ee ku-dhufashada ee ilaalinta iftiinka |
| qiimaha pH | 9.5±0.05 |
| Qiimaha asalka | 200-10000 |
| Qiimaha iftiinka sare ee qiimaha CV (marka la barbar dhigo heerka caadiga ah, xaaladaha falcelintu waxay ahaayeen 150+5μL, xoojinta enzyme waxay ahayd 0.0032U/ml) | ≤10% |
Gaadiidka iyo kaydinta
Gaadiidka:Ambient
Kaydinta:Ku kaydi heerkulka 2-8 ° C, si adag looga ilaaliyo iftiinka
Lagu taliyay in dib loo tijaabiyo Nolosha:2 sano
