APS-5 Chemiluminescence Substrate Solution (Alkaline Phosphatase)
Sharaxaada
Xalka substrate chemiluminescent APS-5 waa jiil cusub oo ah difaaca jirka ee chemiluminescence dareeraha indhaha, alaabtani waa xal isku dhafan oo biyo ku salaysan oo ku salaysan dareeraha isku dhafan ee APS-5, iyada oo loo marayo hagaajinta qaybaha muhiimka ah sida xoojinta, xasiliyayaasha, iyo ilaalinta, waxqabadka ee badeecada si weyn ayaa loo hagaajiyay, waxaana loo isticmaali karaa ficilka fosfatase alkaline (ALP).Dareen-celinta Achemiluminescence waxay dhacdaa iyadoo la isticmaalayo, iyo sawir-qaadayaasha si degdeg ah ayaa loo sii daayaa.Ka dib marka lagu daro muunada Qiimaha ugu badan ee iftiinka leh waxaa lagu gaari karaa qiyaastii 2 daqiiqo, xilliga taaggaduna waxay qaadataa waqti dheer.Badeecadani waxay ku jirtaa kala duwanaanshaha fiirsashada enzyme ALP ee 10-4-10-8 U, Tirada photons ee la sii daayay waxay u dhigantaa fiirsashada ALP ee xalka, oo ku habboon nidaamka difaaca jirka ee Chemiluminescence oo leh ALP sida calaamadaynta enzyme, oo leh It waxay leedahay faa'iidooyinka jawaab celinta degdega ah, dareenka sare, xasiloonida sare, iyo saamiga sare ee signal-to-sanqa.
Isticmaal
Fosfatase alkaline (Alkaline Phosphatase, ALP) oo ah difaaca caadiga ah ee Chemiluminescent ee calaamadaha.Ku habboon iftiinka tubbada, iftiinka saxanka, ogaanshaha chemiluminescence POCT, iwm. isla goobta.
Qaab-dhismeedka Kiimikada
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Sharaxaada | dareere saafi ah oo huruud ah |
| Xasilooni | Qiimaha Iuminescence ee 37℃ ayaa lagu hayaa in ka badan 85% 7 maalmood ka dib markii lagu beeray ilaalinta iftiinka |
| Qiimaha asalka | 500 |
| Qiimaha iftiinka (xaaladda falcelinta ee 150+5μL, qiyaasta enzyme 0.02mU) | 1860000± 5% |
| Ku celcelinta | ≤5% |
Astaamaha Muhiimka ah
Dareenka sare, wuxuu ogaan karaa 10-8 U ama xitaa hoos u dhigista wiilka enzyme ALP.
Qiimaha asalka ahi waa hooseeyaa, qiimaha iftiinka ayaa sarreeya, saamiga calaamad-ilaa-sanqa ayaa sarreeya, qiimaha iftiinka ugu sarreeyana waa la gaareyWaqtigu waa gaaban yahay, falcelinta iftiinka iftiinka ALP waxay gashaa marxaladda taagga gudaha 2 min, iyo luminescence qiimaha ayaa kara degganaansho muddo dheer
Kala duwanaansho toosan oo ballaadhan, xoogga saarista ALP waa 5 amarro oo cabbir ah 10-4-10-8U Qiimaha iftiinka iftiinka ee cabbirku waa xiriir toos ah
Gaadiidka iyo kaydinta
Gaadiidka:Ambient
Kaydinta:Ku kaydi heerkulka 2-8 ° C, si adag looga ilaaliyo iftiinka
Lagu taliyay in dib loo tijaabiyo Nolosha:1 sano
