Recombinant Trypsin
Faa'iidada
1.Asal xoolo ah
2.Nadiif sare
3.Xakamaynta habka wanaagsan
4.Tayada deggan
Sharaxaada
Trypsin waxay si gaar ah u jebisaa C-terminal peptide bonds ee lysine iyo arginine, taas oo hoos u dhigi karta borotiinnada isku xidha unugyadu.Taxanaha amino acid ee trypsin-ka recombinant ee ay soo saarto Hzymes Biotech waa la mid kan porcine-ka ka soo baxa trypsin-ka beeryarada, waxaana soo saaray dib-u-dhaqaajinta Escherichia coli.Waxay u beddeli kartaa soo saarista dhaqameed ee trypsin dheefshiidka unugyada tallaalada, unugyada asliga ah, daawaynta unugyada difaaca, baadhista daroogada, unugyada difaaca jirka iyo qaybaha kale.Aprotinin, soybean trypsin inhibitor, iwm. waxay si cad u joojin karaan dhaqdhaqaaqa trypsin-ka.
Recombinant trypsin waxay leedahay sifooyin la mid ah kan la soo saaro trypsin si dabiici ah.Alaabtan waxa lagu soo saaray aqoon-is-weydaarsiga darajada GMP.Ma jiraan wax asal ah oo xayawaan ah oo loo isticmaalo halsano, nadiifinta iyo maaddooyinka ugu dambeeya.Isla mar ahaantaana, hababka wax soo saarka iyo xakamaynta tayada ayaa la ansaxiyay, taasoo keentay heer sare oo joogto ah oo u dhexeeya kooxo.
Qaab-dhismeedka Kiimikada
Qeexitaan Unug
25 ℃, pH 7.6, 3.2ml xal falcelin (1cm jidka iftiinka), hal unug trypsin (U) ayaa lagu qeexay sida
korodhka 0.003 ee qiimaha nuugista ee 253nm by enzymatic hydrolysis ee BAEE daqiiqadii.
Isticmaalka Alaabta
Dhaqanka unugga
• Dheefshiidka oo xannibaya unugyada, helitaanka unugga aasaasiga ah.
• Dheefshiidka unugyada ku dheggan.
• Dhaqanka unugga ee habka microcarrier.
• Si tartiib ah u dheefshiida unugyada asliga ah.
• Daaweynta unugyada difaaca jirka, iwm.
Loogu talagalay borotiinka recombinant
• Soo saarista insulin-ta dib-u-habaynta.
• Isku xigxiga borotiinka, khariidaynta peptide.
• Nidaamyada u gaarka ah boroteolytiga sida cilmi-baarista borotokoolka
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Muuqashada | Caddaan ama ka baxsan budo cad |
| Solubility (10mg/ml) | Cad xal aan midab lahayn |
| daahirnimo | Β-trypsin ≥70%, α-trypsin ≤ 20% |
| Hawl gaar ah (budada) | ≥2500U/mg |
| E.Coli hadhaaga borotiinka | ≤0.01% |
| Endotoxins (LAL-tijaabada) | ≤20 EU/mg |
Gaadiidka iyo kaydinta
Gaadiidka:Lagu soo raray wax ka yar 2-8 °C
Kaydinta:Ku kaydi heerkulka -2-8°C, ka dib marka lagu milmo 1mM hcl ama 50nM HAC, waa in lagu kaydiyaa ka hooseeya -25 ~ -15 °C
Lagu taliyay in dib loo tijaabiyo Nolosha:2 sano
