
Recombinant Trypsin
Trypsin waxay si gaar ah u jebisaa C-terminal peptide bonds ee lysine iyo arginine, taas oo hoos u dhigi karta borotiinnada isku xidha unugyadu.Taxanaha amino acid-ka trypsin-ka recombinant ee uu soo saaray Hyasen Biotech waa la mid kan boorsiga ee beeryaradu ka soo jeedo trypsin-ka, waxaana soo saaray dib-u-dhaqaajinta Escherichia coli.Waxay bedeli kartaa soo saarista dhaqameed ee trypsin dheefshiidka unugyada tallaalada, unugyada asliga ah, daawaynta unugyada difaaca, baaritaanka daroogada, unugyada difaaca jirka iyo qaybaha kale.Aprotinin, soybean trypsin inhibitor, iwm. waxay si cad u joojin karaan dhaqdhaqaaqa trypsin.
Recombinant trypsin waxay leedahay sifooyin la mid ah kan la soo saaro trypsin si dabiici ah.Alaabtan waxa lagu soo saaray aqoon-is-weydaarsiga darajada GMP.Ma jiraan wax asal ah oo xayawaan ah oo loo isticmaalo halsano, nadiifinta iyo maaddooyinka ugu dambeeya.Isla mar ahaantaana, hababka wax soo saarka iyo xakamaynta tayada ayaa la ansaxiyay, taasoo keentay heer sare oo joogto ah oo u dhexeeya kooxo.
Tilmaamaha alaabta
CAS | 9002-07-7 |
EC | |
Dabeecad | Caddaan ama ka baxsan awoodda crystalline cad |
Miisaanka molecular | 24kDa |
Barta koronto | 8.26 |
Daahirnimada (RP-HPLC) | β-trypsin≥70.0%;α-Trypsin≤20.0% |
Waxqabad gaar ah | ≥3800.0U/mg borotiinka (BAEE) |
Waxqabadka trypsin-ka ee dib-u-dhaqaajinta ah | ≥2500.0U/mg awood (BAEE) |
Aqoonsiga | Purple |
pH ugu fiican | 8.0 |
Culayska noole | ≤300CFU/g |
E. Coli hadhaaga borotiinka unugga martida loo yahay | ≤0.01% |
E. Colihadhaaga unugga martida loo yahay ee DNA | ≤10.0000ng/mg |
Waxa ku jira Endotoxin (LAL-Test) | ≤20.0EU/mg |
Waxa ku jira borotiinka | 95.0%±10.0% |
Xaaladaha kaydinta
Xasiloonida kaydinta: Dib-u-habaynta trypsin lyophilized awoodda waa in lagu kaydiyaa wax ka hooseeya 2-8 ℃.Waxay xasiloon tahay 24 bilood gudahood.Ka dib marka lagu milmo 1mM HCl ama 50mM HAC, waa in lagu kaydiyaa wax ka hooseeya -25 ℃ - 15 ℃ .Ma aha wax hawl lumin ka dib 10 jeer oo soo noqnoqda baraf iyo dhalaalid.
Qeexitaanka unugga
25 ℃ , pH 7.6, 3.2ml xal falcelin (1cm iftiinka wadada), hal unit trypsin (U) ayaa lagu qeexay kororka 0.003 ee qiimaha nuugista at 253nm by enzymatic hydrolysis of BAEE daqiiqadii.
Xakamaynta tayada
Hawsha: Go'aaminta dhaqdhaqaaqa trypsin ee Pharmacopoeia ee Jamhuuriyadda Dadka Shiinaha Volume 4 (Daabacaadda 2020).
.E. Coli martigeliyaha unugga borotiinka haraaga: ELISA Kit.Pharmacopoeia ee Jamhuuriyadda Dadka Shiinaha Volume 4 (Daabacaadda 2020) -E. Coli hadhaaga borotiinka unugga martida loo yahay (3412).
.Nadiifnimada borotiinka: Pharmacopoeia ee Jamhuuriyadda Dadka ee Shiinaha Volume 4 (Daabacaadda 2020) - Xeerarka Guud ee Habka HPLC (0512).
. Culayska noolePharmacopoeia ee Jamhuuriyadda Dadka ee Shiinaha Volume 4 (2020 Edition)- Xeerarka Guud ee Imtixaanka Dhalmada (1101).•E. Coliresidu unugga martida loo yahay ee DNAePharmacopoeia ee Jamhuuriyadda Dadka Shiinaha Volume 4 (Daabcaadda 2020) Hadhaaga unugyada unugga ee DNA (3407).. Endotoxin nuxurkaPharmacopoeia ee Jamhuuriyadda Dadka Shiinaha Volume 4 (2020 Edition) - Xeerarka Guud ee LAL-Test (1143).
Tilmaamaha alaabta
1.Diyaarinta xalalka: Qaado qaddar ku habboon oo trypsin ah iyadoo loo eegayo nuxurka borotiinka (oo lagu xisaabiyo dhaqdhaqaaqa awoodda iyo dhaqdhaqaaqa borotiinka) ee COA, oo ku dar xal dheellitirka HBSS (ama kayd kale oo ku habboon dheefshiidka unugyada. Haddii loo baahdo, ku dar EDTA ugu dambeyntii uruurinta 0-1mM, waxaa fiican in aan ka badnayn 2mM).Feejignaanta trypsin-ka ee lagu taliyay waxay ku saabsan tahay 0. 1-0.3mg/mL (xoojinta ayaa lagu hagaajiyay iyadoo loo eegayo unugyo kala duwan), oo si tartiib ah isku qasan;Tallaabadan waxaa lagu qali karaa heerkulka qolka.
2. Isticmaal filter 0.22μm si aad u shaandhayso xalka trypsin oo aad ugu wareejiso weel nadiif ah;Tallaabadan waxa lagu shaqayn karaa heerkulka qolka.
3. Xalka trypsin ee la sifeeyay waa in si toos ah loo isticmaalo sida loogu baahan yahay maalinta (tusaale, ku darida 1mL ilaa T25 dhalooyinka, unugyada dheefshiidka 37°C), dareerahana waxa lagu kaydin karaa 2-8°C ilaa 1-2 toddobaad. .
4. Kaydinta muddada-dheer: Haddii xalka dheef-shiid kiimikaadka unugga ee la diyaariyey uu u baahan yahay in la kaydiyo wakhti dheer, waxa lagu kaydin karaa si adag at -25~- 15°C muddo 12 bilood ah.
Isticmaalka alaabta
Unug dhaqan
• Dheefshiidka oo xannibaya unugyada, helitaanka unugga aasaasiga ah.
• Dheefshiidka unugyada ku dheggan.
• Dhaqanka unugga ee habka microcarrier.
• Si tartiib ah u dheefshiida unugyada asliga ah.
• Daaweynta unugyada difaaca jirka, iwm.
Loogu talagalay borotiinka dib-u-habaynta:
.Soo saarista insulin-ta dib-u-habaynta.
.Isku xigxiga borotiinka, khariidaynta peptide.
.Nidaamyada proteolytic ee gaarka ah sida cilmi baarista asproteomics.
Ka taxadaritaan
.Xaddiga sarreeya ee milixdu waxay saameyn muuqata ku leedahay hawsha.
.Iska ilaali inaad la xiriirto alaabada kale ee borotiinka.
.Badbaadadaada iyo caafimaadkaaga, fadlan xidho jaakad shaybaadhka iyo galoofyada operatin