Xaddidaadda BsaI Endonuclease

Daahirsanaanta:20U/μL

Xidhmada: 50μL, 250μL, 1ml, 10ml, 100ml

Soo dir weydiin

Faahfaahinta Alaabta

Sharaxaada

Enzyme xaddidaadda BsaI waa nooca II xaddidaadda endonuclease oo laga soo qaatay borotiinka dib-u-dhaqaajinta ee ay ku qoran tahay hidda-wadaha BspQI ee Bacillus sphaericus oo uu muujiyey E.coli.Xakamaynta endonucleases ayaa si weyn loogu isticmaalaa khariidaynta hidda-wadaha, cloning iyo meelaha kale.Enzyme Tani waxay si degdeg ah u gooyaa DNA-da si hufan oo toos ah hidda-wadaha Bsa I , xaddidaadda IIs ee xaddidaadda endonuclease xaddidaadda endonuclease, ayaa laga soo qaatay dib-u-soo-kabashada E. coli oo qaada hidda-wadaha BsaI ee cloned iyo wax laga beddelay ee Bacillus stearothermophilus.kuwaas oo si gaar ah u leh enzymes-ka asalka ah iyo dhaqdhaqaaqa xiddigaha oo yaraaday.Siday isugu xigto aqoonsiga iyo goobaha kala-goynta waa sidan soo socota:

5'······GGTCTC(N)······ ··3'

3'······CCAGAG(NNNN)·······5'

Qaab-dhismeedka Kiimikada

Tilmaamaha alaabta

• Xannibaadda endonuclease waxay leedahay firfircooni enzymatic sare leh waxayna si dhakhso ah u dhamaystirtaa falcelinta dheefshiidka.

• Dhaqdhaqaaqa endonuclease oo hooseeya oo aan gaar ahayn si loo hubiyo goynta saxda ah ee "scalpel".

• Ma jiro nidaam BSA, oo yareynaya wasakhowga isha kulaylka.

Tilmaamid

Walxaha Tijaabada	Tilmaamaha
Dhaqdhaqaaqa Enzyme	20 U/µL
Daahirnimada(SDS-BOG)	≥95%
Rnase	Midna lama ogaan
Waxqabadka DNA-ga Aan Gaarka ahayn	Lama ogaan
Dhaqdhaqaaqa Xiddiga	Lama ogaan
Dib u dhigista iyo dib u dhigista	Dheefshiidka ka dib, jajabyada DNA-da ayaa la isku xiri karaa.Oo jajabyadan isku xidhan waxa lagu gooyn karaa Bsal.
Endotoxins (LAL-tijaabada)	≤10 EU/mg
E.Coli DNA	≤1 Nuqul/2U

Xakamaynta tayada

Nadiifinta ≥ 95%, DNase ma jiro, dhaqdhaqaaqa RNase, Hadhaaga DNA-ga martida loo yahay ≤ 100pg/mg, hadhaaga borotiinka martida loo yahay ≤ -50ppm, hadhaaga endotoxin ≤ 10EU/mg

Codsiga

Nadiifi plasmid-ka si aad u diyaariso jajab DNA oo toosan dhamaadka Poly (A/T/C/G);

Linearize template plasmid ka hor qoraalka in vitro;

Dheefshiidka xaddidan;

Qeexitaan Unug

Hal unug ayaa lagu qeexaa sida qadarka enzyme loo baahan yahay si loo dheefshiido 1 µg oo ah kontoroolka gudaha DNA 1 saac 37°C wadarta mugga falcelinta ee 50 µL