Proteinase K (Budada Lyophiled)
Faa'iidooyinka
● Deganaanshiyo sare iyo firfircoonida ensaymes oo ku salaysan tignoolajiyada kobaca ee la toosay
● Dulqaad u leh milixda Guanidine
● RNase bilaash ah, DNA-da xorta ah iyo Nickase-free, DNA <5 pg/mg
Sharaxaada
Proteinase K waa serine protease deggan oo leh substrate gaar ah.Waxay hoos u dhigtaa borotiinno badan oo ku jira gobolka hooyo xitaa marka ay joogaan saabuunta.Caddaynta laga helay daraasadaha qaab-dhismeedka kristanta iyo molecular waxay tilmaamaysaa insaymku ay iska leeyihiin qoyska subtilisin oo leh goob firfircoon oo firfircoon (Asp 39-His 69-Ser 224).Goobta ugu badan ee jeexjeexa ayaa ah isku-xidhka peptide ee ku dheggan kooxda karboxyl ee asiidhyada amino ee aliphatic iyo caraf udgoon oo leh kooxo alfa amino ah oo xannibmay.Waxaa caadi ahaan loo isticmaalaa gaar ahaan ballaaran.
Qaab dhismeedka kiimikada
Tilmaamid
Walxaha tijaabi | Tilmaamaha |
Sharaxaada | Cadaan ama ka baxsan budada amorphous cad, Lyophilied |
Hawsha | ≥30U/mg |
Solubility (50mg Powder/ml) | Cad |
RNase | Midna lama ogaan |
DNA | Midna lama ogaan |
Nickase | Midna lama ogaan |
Codsiyada
Xirmada ogaanshaha hidda-socodka;
Xirmooyinka soo saarista RNA iyo DNA;
Ka-soo-saarka qaybaha aan borotiinka ahayn ee unugyada,hoos u dhaca wasakhda borotiinka, sida
Tallaalka DNA iyo diyaarinta heparin;
Diyaarinta koromosoomyada DNA-da ee loo yaqaan 'electrophoresis pulsed;
Burburka reer galbeedka;
Enzymatic glycosylated albumin reagents in vitro ogaanshaha
Raridda iyo Kaydinta
Rarida:Ambient
Xaaladaha Kaydinta:Ku kaydi at -20℃(Long term)/2-8℃( Short term)
Taariikhda dib-u-imtixaanka lagu taliyay:2 sano
Ka taxadaritaan
Xidho galoofyada ilaalinta iyo muraayadaha marka aad isticmaalayso ama miisaanayso, si fiicanna hawo u sii isticmaal ka dib.Alaabtani waxay keeni kartaa falcelinta xasaasiyadda maqaarka.Sababta cuncun indhaha oo halis ah.Haddii la nuugo, waxay keeni kartaa xasaasiyad ama calaamadaha neefta ama dyspnea.Waxa laga yaabaa inay keento cuncun xagga neefsiga ah.
Qeexitaanka Cutubka Assay
Hal unug (U) ayaa lagu qeexaa sida xadiga insaymka looga baahan yahay in lagu hydrolyze casein si loo soo saaro 1 μmol tyrosine daqiiqadiiba xaaladaha soo socda.