Ultra Nuclease
Sharaxaada
UltraNuclease waa endonuclaese hidde ahaan loo farsameeyay oo laga soo qaatay Serratia marcescens, kaas oo awood u leh inuu hoos u dhigo DNA ama RNA, labanlaab ama hal xarig, toosan ama wareeg ah oo hoos imaanaya xaalad aad u ballaaran, oo gebi ahaanba hoos u dhigaysa asiidhka nucleic-ka 5'-monophosphate oligonucleotides oo leh 3-5 dhererka salka.
Ka dib markii wax ka beddelka injineernimada hidaha, badeecada ayaa la khamiiriyey, la muujiyey, oo lagu nadiifiyey Escherichia coli (E. coli), taas oo yaraynaysa viscosity ee supernatant unug iyo lysate unug ee cilmi-baarista sayniska, laakiin sidoo kale hagaajinaysaa hufnaanta sifaynta iyo cilmi-baarista functional ee borotiinka.Waxa kale oo loo isticmaali karaa daaweynta hidda-wadaha, nadiifinta fayraska, soosaarka tallaalka, borotiinka iyo warshadaha dawooyinka ee polysaccharide sida reagent ka saarista aashitada nucleic ee martida loo yahay.
Qaab-dhismeedka Kiimikada
Qeexitaan Unug
Qadarka enzyme loo isticmaalo in lagu beddelo qiimaha nuugista ee △A260 by 1.0 gudaha 30min at 37 °C, pH 8.0, oo u dhiganta 37μg salmon shahwada dheefshiidka DNA by gooyay oligonucleotides, waxaa lagu qeexay sida unug firfircoon.
Isticmaalka iyo qiyaasta
• Ka saar aashitada nucleic-ka-baxsan ee alaabta tallaalka, yareyso halista haraaga sunta nucleic-ka iyo hagaajinta badbaadada alaabta.
• Yaree viscosity ee dareeraha quudinta ee ay keento nucleic acid, yarayso wakhtiga habaynta iyo kordhiso dhalidda borotiinka.
• Ka saar aashitada nucleic-ka ah ee ku duudduubtay qayb (fayras, jidhka ku darida, iwm.), kaas oo ku habboon sii daynta iyo sifaynta qaybta.
• Daawaynta Nukliyeerku waxay wanaajin kartaa xallinta iyo soo kabashada muunadda ee chromatography tiirka, electrophoresis iyo falanqaynta xoqidda.
• Daawaynta hidda-wadaha, aashitada nucleic ayaa laga saaraa si loo helo fayrasyo la safeeyey adeno oo la safeeyey.
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Sharaxaada | Cad oo aan midab lahayn |
| Hawsha | ≥ 250 U/ul |
| Dhaqdhaqaaq gaar ah | ≥1.1*106U/mg |
| Daahirnimada (SDS-PAGE) | ≥ 99.0% |
| borotiinka | Midna lama ogaan |
| Culayska noole | 10 cfu/100,000U |
| Endotoxines (LAL-tijaabada) | 0.25EU/1,000U |
Gaadiidka iyo kaydinta
Gaadiidka:Lagu soo raray wax ka yar 0 °C
Kaydinta:Ku kaydi heerkulka -25 ~ -15°C
Lagu taliyay in dib loo tijaabiyo Nolosha:2 sano (iska ilaali qaboojinta - dhalaalidda)
