D-Lactate dehydrogenase (LDH)
Sharaxaada
Enzyme waxaa loo isticmaalaa ka saarista pyruvate ee go'aaminta la shaqeynta NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Isticmaalka
1. Fosfatase alkaline oo ay weheliyaan borotiinno (ka-hortagga unugyada, streptavidin iwm,) ayaa si gaar ah u aqoonsan kara molecules bartilmaameedka, waxaana loo isticmaali karaa ELISA, WB iyo ogaanshaha histochemical;
2.Fosphatase alkaline waxaa loo isticmaali karaa in lagu nadiifiyo 5 '-terminal DNA ama RNA si looga hortago isku-xirnaanta;
3.DNA ama RNA-ga kor ku xusan ee dephosphorylated waxa lagu calaamadin karaa fosfooraska raadiyaha ku suntan (iyada oo loo sii marayo T4 poly-nucleotide kinase)
Qaab-dhismeedka Kiimikada
Habka falcelinta
D-Lactate + NAD+→ Pyruvate +NADH + H+
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Sharaxaada | Budada amorphous cad, lyophilized |
| Hawsha | ≥200U/mg |
| Daahirnimada(SDS-BOG) | ≥90% |
| Solubility (10mg budo/ml) | Cad |
| NADH/NADPH oxidase | ≤0.01% |
| Malate fuuqbaxa | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Gaadiidka iyo kaydinta
Gaadiidka:Deegaanka
Kaydinta:Ku kaydi heerkulka -20°C (mudda dheer), 2-8°C (muddada gaaban)
Dib-u-imtixaan lagu taliyayNolosha:2 sano
