KIT ELEISA ee Trypsin
Sharaxaada
Recombinant Trypsin waxaa inta badan loo isticmaalaa wax soo saarka biopharmaceutical-inta lagu jiro diyaarinta unugyada ama wax ka beddelka iyo hawlgelinta alaabta.Trypsin waxay keenaysaa khataro nabdoon, sidaas awgeedna waa in meesha laga saaraa ka hor inta aan la sii deyn badeecada u dambaysa.Qalabka Sandwich-ka waxa loogu talagalay cabbiraadda tirada haraaga
Xirmadani waa Enzyme-Linked Immunosorbent Assay (ELISA).Saxanka waxa hore loogu dahaadhay Porcine trypsin antibody.Trypsin-ka ku jirta muunada ayaa lagu daraa oo ku xidhaa unugyada difaaca jirka ee lagu dahaadhay ceelasha.Ka dibna biotinylated Porcine trypsin antibody ayaa lagu daraa oo ku xidhaa trypsin ka muunada.Ka dib marka la dhaqo, HRP-Streptavidin ayaa lagu daraa oo ku xidhaa antibody-ka biotinylated trypsin.Ka dib marka la isku xidho HRP-Streptavidin waa la dhaqay.Kadibna xalka TMB substrate ayaa lagu daraa oo ay kicisay HRP si ay u soo saarto badeeco midab buluug ah oo isu beddeshay huruud ka dib marka lagu daro xal joojinta acidic.Cufnaanta jaalaha ah waxay u dhigantaa qaddarka la beegsanayo ee trypsin
muunad lagu qabtay saxan.Nuugista waxaa lagu qiyaasaa 450 nm.
Qaab-dhismeedka Kiimikada
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Muuqashada | Baakad dhammaystiran oo wax dareere ah ma daadin |
| Xadka hoose ee ogaanshaha | 0.003 ng/ml |
| Xadka hoose ee tirada | 0.039 ng/ml |
| Saxnaanta | Baaritaanka gudaha CV≤10% |
Gaadiidka iyo kaydinta
Gaadiidka:Ambient
Kaydinta:Waxa lagu kaydin karaa heerkulka -25 ~ -15°C nolosha shelf, 2-8°C si loogu fududeeyo tijaabada kale
Lagu taliyay in dib loo tijaabiyo Nolosha:1 sano
