Kolestarool oxidase (COD/CHOD)
Sharaxaada
Kolesteroolka oxidase (CHOD) wuxuu kiciyaa tallaabada ugu horreysa ee catabolismka kolestaroolka.Qaar ka mid ah bakteeriyada aan bakteeriyada ahayn, sida Streptomyces ayaa awood u leh inay ka faa'iidaystaan kolestaroolka isha kaarboonka.Bakteeriyada cudur-sidaha leh, sida Rhodococcus equi, waxay u baahan tahay CHOD si ay u qaadsiiso makrophage-ka martida loo yahay.CHOD waa laba-shaqaale. Kolestaroolka ayaa markii hore oksaydhiyay kolestarool-5-en-3-hal tallaabo FAD u baahan.Cholest-5-en-3-one waa la soocay ilaa cholest-4-en3-one.Hawsha CHOD waxay ku xidhan tahay sifooyinka jidheed ee xuubka uu substrate-ku ku xidhan yahay.
CHOD waxaa loo isticmaalaa in lagu go'aamiyo kolestaroolka serum.Waa enzyme labaad ee loogu isticmaalka badan yahay codsiyada ogaanshaha ka dib glucose oxidase.CHOD waxay sidoo kale ka heleysaa codsiga microanalysis ee steroids ee muunadaha cuntada iyo kala soocida 3-ketosteroids ee 3b-hydroxysteroids. Dhirta transgenic ee muujinaya kolestaroolka oxidase ayaa lagu baarayaa dagaalka ka dhanka ah weevil suufka.Kolestarool oxidase ayaa sidoo kale loo isticmaalay sidii baaritaan molecular si loo caddeeyo qaababka xuubabka gacanta.
Qaab-dhismeedka Kiimikada
Mabda'a Falcelinta
Kolestaroolka + O2 →△4-Cholesten-3-hal + H2O2
Tilmaamid
| Walxaha Tijaabada | Tilmaamaha |
| Sharaxaada | budada amorphous jaalaha ah, lyophilized |
| Hawsha | ≥8U/mg |
| Daahirnimada(SDS-BOG) | ≥90% |
| Solubility (10mg budo/ml) | Cad |
| Catalase | ≤0.001% |
| Glucose oxidase | ≤0.01% |
| Cholesterol sterase | ≤0.01% |
| ATPase | ≤0.005% |
Gaadiidka iyo kaydinta
Gaadiidka:Raray ka hooseeya -15 ° C
Kaydinta:Ku kaydi heerkulka -25 ~ -15 ° C (mudda dheer), 2-8 ° C (wakhti gaaban)
Dib-u-imtixaan lagu taliyayNolosha:1 sano
